del Valle Kara, Theus Michelle H, Bethea John R, Liebl Daniel J, Ricard Jerome
Department of Pediatrics, Division of Neonatology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Int J Dev Neurosci. 2011 Feb;29(1):9-14. doi: 10.1016/j.ijdevneu.2010.10.005. Epub 2010 Oct 20.
The subventricular zone (SVZ) of the mammalian forebrain is a major source of multipotent stem cells during development, and contributes to neurogenesis throughout the lifespan of the organism. Several studies described molecules regulating adult neurogenesis, however, few of them have examined neurogenesis in the early postnatal period. Adult neurogenesis is regulated in part by ephrinB3 and its receptors, so we examined the role of EphB3 on neural stem/progenitor cell (NSPC) proliferation in early postnatal development in the SVZ. To examine NSPC proliferation, we used BrdU incorporation in both cultured NSPCs and neonatal gene-targeted knockout mice, as well as Ki67 immunostaining in EphB3(-/-) mice. We observed a significant increase in proliferation in cultured NSPCs derived from EphB3(-/-) mice and in the SVZ of EphB3(-/-) mice. These studies support an anti-proliferative role for EphB3 in regulating NSPC numbers in the developing SVZ.
哺乳动物前脑的脑室下区（SVZ）是发育过程中多能干细胞的主要来源，并在生物体的整个生命周期中对神经发生有贡献。几项研究描述了调节成体神经发生的分子，然而，其中很少有研究在出生后早期检查神经发生。成体神经发生部分受ephrinB3及其受体调节，因此我们研究了EphB3在出生后早期SVZ发育中对神经干细胞/祖细胞（NSPC）增殖的作用。为了检查NSPC增殖，我们在培养的NSPC和新生基因靶向敲除小鼠中使用了BrdU掺入，以及在EphB3（-/-）小鼠中进行Ki67免疫染色。我们观察到源自EphB3（-/-）小鼠的培养NSPC和EphB3（-/-）小鼠的SVZ中增殖显著增加。这些研究支持EphB3在调节发育中的SVZ中NSPC数量方面具有抗增殖作用。
