Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
J Chromatogr A. 2010 Nov 26;1217(48):7478-83. doi: 10.1016/j.chroma.2010.09.079. Epub 2010 Oct 7.
A simple, rapid and sensitive method for the determination of pirimicarb in tomato and pear using polymer monolith microextraction (PMME) based on the molecularly imprinted polymer (MIP) monolith combined with high-performance liquid chromatography-photodiodes array detector (HPLC-PAD) was developed. By optimizing the polymerization conditions, such as the nature of porogenic solvent and functional monomer, the molar ratio of the monomer and cross-linker, an pirimicarb MIP monolith was synthesized in a micropipette tip using methacrylic acid (MAA) as the functional monomer, ethylene dimethacrylate (EGDMA) as the cross-linker and the mixture of toluene-dodecanol as the porogenic solvent. The MIP monolith showed highly specific recognition for the template pirimicarb. The monolith was applied for the selective extraction of pirimicarb in tomato and pear. Several parameters affecting MIP-PMME were investigated, including the nature and volume of extraction solvent, sample volume, flow rate and sample pH. Under the optimum PMME and HPLC conditions, the linear ranges were 2.0-1400 μg/kg for pirimicarb in tomato and pear with the correlation coefficient of above 0.999. The detection limits (s/n=3) were both 0.6 μg/kg. The proposed method was successfully applied for the selective extraction and determination of pirimicarb in tomato and pear.
一种基于分子印迹聚合物（MIP）整体柱的聚合物整体微萃取（PMME）结合高效液相色谱-光电二极管阵列检测器（HPLC-PAD）的简单、快速、灵敏的测定番茄和梨中咪鲜胺的方法。通过优化聚合条件，如致孔溶剂和功能单体的性质、单体和交联剂的摩尔比，在微量进样管中合成了以甲基丙烯酸（MAA）为功能单体、乙二甲基丙烯酸（EGDMA）为交联剂、甲苯-十二醇混合物为致孔溶剂的咪鲜胺 MIP 整体柱。MIP 整体柱对模板咪鲜胺表现出高度的特异性识别。该整体柱用于番茄和梨中咪鲜胺的选择性萃取。考察了影响 MIP-PMME 的几个参数，包括萃取溶剂的性质和体积、样品体积、流速和样品 pH 值。在最佳 PMME 和 HPLC 条件下，咪鲜胺在番茄和梨中的线性范围分别为 2.0-1400μg/kg，相关系数均大于 0.999。检测限（s/n=3）均为 0.6μg/kg。该方法成功应用于番茄和梨中咪鲜胺的选择性提取和测定。
