Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.
Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2314-2319. doi: 10.1099/ijs.0.021410-0. Epub 2010 Oct 22.
Here, we describe a novel bacterium, strain TUD-YJ37(T), which can accumulate polyhydroxybutyrate (PHB) to more than 85 % (w/w) dry cell weight. The bacterium was isolated from a mixed-culture bioreactor by using a feast-famine regime and its properties were characterized. Phylogenetic analysis based on full 16S rRNA gene sequences revealed that the isolate is a member of the Gammaproteobacteria, forming an independent, deep phylogenetic lineage. It is most closely related to members of the genera Methylocaldum, Methylococcus and Natronocella, with sequence similarities below 91 %. Strain TUD-YJ37(T) was an obligately aerobic, ovoid, Gram-negative bacterium, motile by means of a polar flagellum. It utilized C₂-C₁₀ fatty acids as carbon and energy sources. The temperature range for growth was 20-35 °C, with an optimum of 30 °C; the pH range was 6.0-8.0, without a clear optimum. The major respiratory quinone was Q-8. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, an unidentified aminolipid and another unidentified lipid. The predominant fatty acids in the membrane polar lipids were C₁₆:₁ω7c, C₁₆:₀ and C₁₈:₁ω7c. The G+C content of the genomic DNA was 67.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, the isolate is proposed to represent a novel genus and species, for which the name Plasticicumulans acidivorans gen. nov., sp. nov. is proposed. The type strain of Plasticicumulans acidivorans is TUD-YJ37(T) ( = DSM 23606(T) = CBS 122990(T)).
这里，我们描述了一株新型细菌，菌株 TUD-YJ37(T)，它可以将聚羟基丁酸酯 (PHB) 积累到超过 85%（干重）。该细菌是通过采用丰度变化培养方式从混合培养生物反应器中分离得到的，其特性已被表征。基于全长 16S rRNA 基因序列的系统发育分析表明，该分离株是γ变形菌的成员，形成一个独立的、深系统发育分支。它与 Methylocaldum、Methylococcus 和 Natronocella 属的成员最为密切相关，序列相似性低于 91%。菌株 TUD-YJ37(T) 是一种需氧、卵形、革兰氏阴性细菌，通过极生鞭毛运动。它利用 C₂-C₁₀ 脂肪酸作为碳源和能源。生长的温度范围为 20-35°C，最适温度为 30°C；pH 值范围为 6.0-8.0，没有明显的最适 pH 值。主要的呼吸醌是 Q-8。极性脂由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未鉴定的磷脂、一种未鉴定的胺脂和另一种未鉴定的脂组成。膜极性脂中的主要脂肪酸是 C₁₆:₁ω7c、C₁₆:₀ 和 C₁₈:₁ω7c。基因组 DNA 的 G+C 含量为 67.4 mol%。基于表型、化学分类学和分子数据，该分离株代表一个新属和新种，建议将其命名为嗜酸塑料球菌属 Plasticicumulans acidivorans gen. nov.，sp. nov.。嗜酸塑料球菌的模式株为 TUD-YJ37(T)（=DSM 23606(T)=CBS 122990(T)）。
