在大肠杆菌中，由 MazF 产生的无帽 mRNA 特异性核糖体进行无帽 mRNA 的选择性翻译。
Selective translation of leaderless mRNAs by specialized ribosomes generated by MazF in Escherichia coli.
机构信息
Max F. Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr. Bohrgasse 9/4, 1030 Vienna, Austria.
出版信息
Cell. 2011 Sep 30;147(1):147-57. doi: 10.1016/j.cell.2011.07.047. Epub 2011 Sep 22.
Abstract
Escherichia coli (E. coli) mazEF is a stress-induced toxin-antitoxin (TA) module. The toxin MazF is an endoribonuclease that cleaves single-stranded mRNAs at ACA sequences. Here, we show that MazF cleaves at ACA sites at or closely upstream of the AUG start codon of some specific mRNAs and thereby generates leaderless mRNAs. Moreover, we provide evidence that MazF also targets 16S rRNA within 30S ribosomal subunits at the decoding center, thereby removing 43 nucleotides from the 3' terminus. As this region comprises the anti-Shine-Dalgarno (aSD) sequence that is required for translation initiation on canonical mRNAs, a subpopulation of ribosomes is formed that selectively translates the described leaderless mRNAs both in vivo and in vitro. Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli.
摘要
大肠杆菌（E. coli）mazEF 是一种应激诱导的毒素-抗毒素（TA）模块。毒素 MazF 是一种内切核糖核酸酶，可在 ACA 序列处切割单链 mRNA。在这里，我们表明 MazF 在一些特定 mRNA 的 AUG 起始密码子处或其附近的 ACA 位点切割，从而产生无 5' 帽 mRNA。此外，我们提供的证据表明，MazF 还靶向核糖体 30S 亚基中解码中心的 16S rRNA，从而从 3' 末端去除 43 个核苷酸。由于该区域包含反 Shine-Dalgarno（aSD）序列，该序列对于经典 mRNA 的翻译起始是必需的，因此形成了一个亚基核糖体，该核糖体选择性地在体内和体外翻译所述无 5' 帽 mRNA。因此，我们发现了一种经过修饰的翻译机制，它是响应 MazF 诱导产生的，可能有助于大肠杆菌的应激适应。
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