Department of Nanoengineering, University of California San Diego, La Jolla, CA 92093, USA.
Nat Commun. 2010 Jul 13;1:36. doi: 10.1038/ncomms1035.
Synthetic nanomotors, which convert chemical energy into autonomous motion, hold considerable promise for diverse applications. In this paper, we show the use of synthetic nanomotors for detecting DNA and bacterial ribosomal RNA in a fast, simple and sensitive manner. The new motion-driven DNA-sensing concept relies on measuring changes in the speed of unmodified catalytic nanomotors induced by the dissolution of silver nanoparticle tags captured in a sandwich DNA hybridization assay. The concentration-dependent distance signals are visualized using optical microscopy, particularly through straight-line traces by magnetically aligned 'racing' nanomotors. This nanomotor biodetection strategy could be extended to monitor a wide range of biomolecular interactions using different motion transduction schemes, thus providing a versatile and powerful tool for detecting biological targets.
合成纳米马达可以将化学能转化为自主运动，在多个领域具有广阔的应用前景。本文展示了如何利用合成纳米马达快速、简便、灵敏地检测 DNA 和细菌核糖体 RNA。这种基于运动的新型 DNA 传感概念依赖于测量未修饰的催化纳米马达速度的变化，这些纳米马达的速度变化是由三明治 DNA 杂交分析中捕获的银纳米颗粒标签的溶解引起的。通过光学显微镜，特别是通过磁性对齐的“竞速”纳米马达的直线轨迹，可以可视化浓度依赖性的距离信号。这种纳米马达生物检测策略可以扩展到使用不同的运动转换方案来监测广泛的生物分子相互作用，从而为检测生物靶标提供了一种通用且强大的工具。
