Departamento de Biociências, Universidade Federal de São Paulo, São Paulo, Brazil.
Cell Biochem Funct. 2010 Dec 2;28(8):632-6. doi: 10.1002/cbf.1700.
The aim of this study was to investigate the effects of acute exercise on genomic damage in an animal model. Male adult Wistar rats were divided into the following groups: control and acute exercised (experimental). For this purpose, 15 animals were accustomed to running on a rodent treadmill for 15 min per day for 5 days (10-20 m min(-1); 08 grade). After 4 days at rest, active animals ran on the treadmill (22 m min(-1), 58 grade) till exhaustion. Cells from peripheral blood, liver, heart, and brain were collected after 0, 2, and 6 h after exercise. The results showed that acute exercise was able to induce genetic damage in peripheral blood cells after 2 and 6 h of exercise, whereas liver pointed out genetic damage for all periods evaluated. No genetic damage was induced either in brain or in heart cells. In conclusion, our results suggest that acute exercise could contribute to the genetic damage in peripheral blood and liver cells. It seems that liver is a sensitive organ to the genotoxic insult after acute exercise.
本研究旨在探讨动物模型中急性运动对基因组损伤的影响。雄性成年 Wistar 大鼠被分为以下几组：对照组和急性运动组（实验组）。为此，15 只动物习惯于每天在老鼠跑步机上跑步 15 分钟，速度为 10-20 m/min，坡度为 08。经过 4 天的休息后，活跃的动物在跑步机上以 22 m/min，坡度为 58 的速度一直跑到筋疲力尽。运动后 0、2 和 6 小时采集外周血、肝脏、心脏和大脑的细胞。结果表明，急性运动在运动后 2 和 6 小时可诱导外周血细胞核遗传损伤，而肝脏在所有评估的时期均显示出遗传损伤。大脑或心脏细胞均未诱导遗传损伤。总之，我们的结果表明，急性运动可能导致外周血和肝细胞的遗传损伤。似乎肝脏是急性运动后对遗传毒性损伤敏感的器官。
