Huang Sheng-Teng, Pang Jong-Hwei S, Yang Rong-Chi
Department of Chinese Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Chang Gung Med J. 2010 Sep-Oct;33(5):477-87.
Phyllanthus urinaria (P. urinaria), a widely used herbal medicine, has been reported to possess various biological activities. This report aimed to characterize the whole P. urinaria plant, present the anticancer effects of P. urinaria both in vivo and in vitro, and explore relevant mechanisms. The water extract of P. urinaria not only significantly reduces the cell viability of various cancer cell lines from different origins but also suppresses tumor development in C57BL/6J mice after implantation of Lewis lung carcinoma (LCC) cells. The anticancer activity of P. urinaria extract is mainly due to induced apoptosis of cancer cells as demonstrated by DNA fragmentation and increased caspase-3 activity through both intrinsic and extrinsic pathways. The decrease in viability with P. urinaria treatment might be partially associated with down-regulation of telomerase activation and induction of the apoptotic process. In addition, P. urinaria also exhibits anti-angiogenic activity that is mediated, at least in part, by suppression of matrix metalloproteinase 2 (MMP-2) secretion and inhibition of MMP-2 activity through zinc chelation.
叶下珠是一种广泛使用的草药，据报道具有多种生物活性。本报告旨在对叶下珠全株进行表征，呈现叶下珠在体内和体外的抗癌作用，并探索相关机制。叶下珠水提取物不仅能显著降低来自不同来源的各种癌细胞系的细胞活力，还能抑制Lewis肺癌（LCC）细胞植入后C57BL/6J小鼠的肿瘤发展。叶下珠提取物的抗癌活性主要归因于诱导癌细胞凋亡，这通过DNA片段化以及通过内源性和外源性途径增加的半胱天冬酶-3活性得以证明。用叶下珠处理后活力的降低可能部分与端粒酶激活的下调和凋亡过程的诱导有关。此外，叶下珠还表现出抗血管生成活性，这至少部分是通过抑制基质金属蛋白酶2（MMP-2）的分泌和通过锌螯合抑制MMP-2活性介导的。
