Liu Feng-huan, Li Ming-yuan, Luo Jun, Li Wan-yi, Wamg Bao-ning, Yang Yuan, Kuang Yu, Zuo Bin, Ma Xiu-ying, Jiang Zhong-hua
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhonghua Yi Xue Za Zhi. 2010 Jul 13;90(26):1863-5.
To study the anti-influenza A virus effects of traditional Chinese medicine Kanggan granules in chicken embryo and BALB/c mice.
The influenza A virus (H(1)N(1), FM1) was used in the experiments. FM1 was cultured in chicken embryo and the anti-FM1 activity of Kanggan granules was evaluated through the post-medication hemagglutination titer of FM1. In animal test, 120 healthy BALB/c mice were randomly divided into six groups, normal control, virus control, ribavirin, high-dosage, middle-dosage and low-dosage. The FM1 infection model was established by dripping FM1 into nasal cavity and then the appropriate treatments were prescribed. The effective anti- FM1 indices of Kanggan granules included survival status, protective percentage of death and life elongation percentage of mice infected with FM1.
The high and middle doses of Kanggan granules could inhibit the replication of FM1 remarkably in chicken embryo, and could reduced hemagglutination titer to 5 and 3 times. In animal experiments, all mice treated with Kanggan granules could improve the general status of infected mice, the protective percentages of death were 35.0% to 55.0%, the life elongation percentages were 73.0% to 88.9% and the minimal effective dose was 3.00 g/kg.
Kanggan granules can inhibit the replication of influenza A virus and protect the mice infected with FM1.
研究中药抗感颗粒对鸡胚和BALB/c小鼠的抗甲型流感病毒作用。
实验采用甲型流感病毒（H(1)N(1)，FM1）。FM1在鸡胚中培养，通过给药后FM1的血凝滴度评估抗感颗粒的抗FM1活性。在动物实验中，将120只健康BALB/c小鼠随机分为六组，即正常对照组、病毒对照组、利巴韦林组、高剂量组、中剂量组和低剂量组。通过滴鼻FM1建立FM1感染模型，然后给予相应处理。抗感颗粒的有效抗FM1指标包括感染FM1小鼠的生存状态、死亡保护率和生命延长率。
抗感颗粒高、中剂量能显著抑制鸡胚中FM1的复制，使血凝滴度分别降低至5倍和3倍。在动物实验中，所有用抗感颗粒治疗的小鼠均可改善感染小鼠的一般状况，死亡保护率为35.0%至55.0%，生命延长率为73.0%至88.9%，最小有效剂量为3.00 g/kg。
抗感颗粒能抑制甲型流感病毒的复制，保护感染FM1的小鼠。
