幽门螺杆菌可塑性区基因与高发地区胃十二指肠疾病的关系。
Associations between the Plasticity Region Genes of Helicobacter pylori and Gastroduodenal Diseases in a High-Prevalence Area.
机构信息
Department of Medicine, The Aga Khan University, Karachi, Pakistan.
出版信息
Gut Liver. 2010 Sep;4(3):345-50. doi: 10.5009/gnl.2010.4.3.345. Epub 2010 Sep 24.
BACKGROUND/AIMS: Genes associated with the Helicobacter pylori (H. pylori) plasticity region may play a role in the pathogenesis of H. pylori. We compared the genes jhp0940, jhp0947, and jhp0986 in H. pylori isolates from patients with different gastroduodenal diseases and in different age groups.
METHODS
The H. pylori hyperplasticity region genes jhp0940, jhp0947, and jhp0986 were studied by PCR. We also evaluated whether these genes were related to the cytotoxin-associated gene (cagA) and histology findings.
RESULTS
Of the patient cohort, 71 (62%) were positive for jhp0940, 67 (59%) for jhp0947, 12 (10%) for jhp0986, and 69 (60%) for cagA. jhp0940 (n=18, 67%) and jhp0947 (n=23, 85%) were found more frequently in duodenal ulcer (DU) patients than in gastritis patients (n=14, 39%; p=0.029 and p<0.001, respectively). Gastric ulcer (GU) was more frequently associated with jhp0940 (17 patients, 77%; p=0.003) than with gastritis (14 patients, 39%). Gastric carcinoma (GC) was more strongly associated with both jhp0940 (22 patients, 76%; p=0.003) and jhp0947 (22 patients, 76%; p=0.003) than was gastritis (14 patients, 39%). jhp0947 was more frequently associated with chronic active inflammation (58 patients, 87%; p=0.009) than with chronic inflammation (9 patients, 13%). Multivariate analysis demonstrated that jhp0947 was associated with DU (odds ratio, 6.1; 95% confidence interval, 1.87-20).
CONCLUSIONS
The genes jhp0947 and jhp0940 were identified in H. pylori isolates from patients with GC and DU, while jhp0940 was also isolated from patients with GU. jhp0947 was independently associated with DU.
背景/目的：与幽门螺杆菌（H. pylori）可塑性区域相关的基因可能在 H. pylori 的发病机制中发挥作用。我们比较了来自不同胃肠疾病患者和不同年龄组的 H. pylori 分离株中的 jhp0940、jhp0947 和 jhp0986 基因。
方法
通过 PCR 研究了 H. pylori 过度增生区基因 jhp0940、jhp0947 和 jhp0986。我们还评估了这些基因是否与细胞毒素相关基因（cagA）和组织学发现有关。
结果
在患者队列中，71 例（62%）为 jhp0940 阳性，67 例（59%）为 jhp0947 阳性，12 例（10%）为 jhp0986 阳性，69 例（60%）为 cagA 阳性。十二指肠溃疡（DU）患者中 jhp0940（n=18，67%）和 jhp0947（n=23，85%）的检出率高于胃炎患者（n=14，39%；p=0.029 和 p<0.001）。胃溃疡（GU）与 jhp0940 的相关性更强（17 例，77%；p=0.003），而与胃炎（14 例，39%）相比。胃癌（GC）与 jhp0940（22 例，76%；p=0.003）和 jhp0947（22 例，76%；p=0.003）的相关性均强于胃炎（14 例，39%）。jhp0947 与慢性活动性炎症（58 例，87%；p=0.009）的相关性强于慢性炎症（9 例，13%）。多变量分析表明，jhp0947 与 DU 相关（比值比，6.1；95%置信区间，1.87-20）。
结论
在 GC 和 DU 患者的 H. pylori 分离株中发现了 jhp0947 和 jhp0940 基因，而 jhp0940 也分离自 GU 患者。jhp0947 与 DU 独立相关。
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