Huang J L
Department of Parasitology, Sun Yat-sen University of Medical Sciences.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(4):253-5.
Observations were carried out on the changes in the susceptibility of Aedes aegypti (refm) to Brugia pahangi after receiving haemolymph from both refractory (repRR) and susceptible (refm) Aedes aegypti that were previously inoculated with microfilariae plus TC199 (tissue culture 199) or TC199 respectively. The melanization rates of microfilariae in the recipient mosquitoes were determined by dissecting the mosquitoes on day 3, 4 and 5 after haemolymph transfer. In the recipients receiving haemolymph from the refractory donors inoculated with microfilariae plus TC199, from the refractory donors inoculated with TC199 only and from the susceptible donors inoculated with TC199 only, the melanization rates of microfilariae were estimated to be 31.2%, 31.1% and 21.2% respectively. It is suggested that the intensity of melanization of mosquitoes varies with their susceptibility inherited from their parents. Therefore, the melanization rates of microfilariae in the mosquitoes can be taken as one of the indices of their susceptibility. In addition, most (64.3-73.6%) of the recovered melanized microfilariae were found in the abdomen of the mosquitoes while almost all the developing larvae, in the thorax, possibly due to the choice of preferred sites for development in the latter.
对埃及伊蚊（refm）在分别接受先前接种过微丝蚴加TC199（组织培养基199）或仅接种TC199的抗性（repRR）和易感（refm）埃及伊蚊的血淋巴后，对彭亨布鲁线虫易感性的变化进行了观察。在血淋巴转移后的第3、4和5天解剖蚊子，以确定受体蚊子体内微丝蚴的黑化率。在接受来自接种微丝蚴加TC199的抗性供体、仅接种TC199的抗性供体以及仅接种TC199的易感供体的血淋巴的受体中，微丝蚴的黑化率估计分别为31.2%、31.1%和21.2%。结果表明，蚊子的黑化强度随其从亲本遗传的易感性而变化。因此，蚊子体内微丝蚴的黑化率可作为其易感性的指标之一。此外，回收的黑化微丝蚴大部分（64.3 - 73.6%）在蚊子腹部被发现，而几乎所有发育中的幼虫在胸部，这可能是由于后者选择了更适宜的发育部位。
