Baskar J F, Stanat S C, Sulik K K, Huang E S
J Infect Dis. 1983 Nov;148(5):836-43. doi: 10.1093/infdis/148.5.836.
The effects of congenital infection with cytomegalovirus on fetal development were studied by injection of murine cytomegalovirus (MCMV) into the endometrial lumina of mice in the early stages of pregnancy. The mice were later killed, and the sites of embryonic implantation were examined. Litter size was reduced and the incidence of abnormal fetuses was significantly increased among MCMV-infected animals. Morbid, moribund, severely growth-retarded, asynchronously developed, and malformed fetuses were found. Virus was reisolated from infected mothers, placentas, and fetal tissues but not from control animals that had received either heat-inactivated virus or culture medium. The recovered virus was identical to the original virus, as verified by restriction enzyme analysis. MCMV-specific DNA was detected in experimental tissue sections by in situ nucleic-acid hybridization. Seroconversion occurred in infected female mice. Thus, the presence of MCMV in the genital tract at the time of embryonic implantation causes not only embryonic infection but also fetal maldevelopment.
通过在妊娠早期将鼠巨细胞病毒（MCMV）注射到小鼠子宫内膜腔中，研究了先天性巨细胞病毒感染对胎儿发育的影响。随后处死小鼠，并检查胚胎着床部位。在感染MCMV的动物中，窝仔数减少，异常胎儿的发生率显著增加。发现了病态、濒死、严重生长迟缓、发育不同步和畸形的胎儿。从感染的母体、胎盘和胎儿组织中重新分离出病毒，但在接受热灭活病毒或培养基的对照动物中未分离出病毒。经限制性酶分析验证，回收的病毒与原始病毒相同。通过原位核酸杂交在实验组织切片中检测到MCMV特异性DNA。感染的雌性小鼠发生了血清学转换。因此，胚胎着床时生殖道中MCMV的存在不仅会导致胚胎感染，还会导致胎儿发育不良。
