气相色谱-质谱法测定尿液中的吗啡和可待因

Determination of morphine and codeine in urine by gas chromatography-mass spectrometry.

作者信息

Solans A, de la Torre R, Segura J

机构信息

Department of Pharmacology and Toxicology, Institut Municipal d'Investigació Mèdica, Barcelona, Spain.

出版信息

J Pharm Biomed Anal. 1990;8(8-12):905-9. doi: 10.1016/0731-7085(90)80140-k.

DOI:10.1016/0731-7085(90)80140-k

PMID:2100640

Abstract

GC-MS is one of the recommended analytical techniques for the identification and confirmation of opiates in urine. A method for the qualitative detection and quantitation of codeine and morphine in urine samples by this technique has been developed. This method is also suitable for the detection of their main metabolites in urine: norcodeine and normorphine. It also allows the identification of 6-monoacetylmorphine in urine, which can be used as a confirmatory marker of heroine abuse. The derivatized compounds are separated by capillary gas chromatography (GC) and identified by mass spectrometry (MS) in the selective ion monitoring acquisition mode (SIM). The recoveries from urine at concentrations of 1000 ng ml-1 are 72% for codeine and 80% for morphine. The method is linear in the range studied (0-1000 ng ml-1) for codeine and morphine.

摘要

气相色谱-质谱联用（GC-MS）是尿液中阿片类药物鉴定和确证的推荐分析技术之一。已开发出一种利用该技术对尿液样本中可待因和吗啡进行定性检测和定量分析的方法。该方法也适用于检测尿液中它们的主要代谢物：去甲可待因和去甲吗啡。它还能鉴定尿液中的6-单乙酰吗啡，其可作为海洛因滥用的确证标志物。衍生化后的化合物通过毛细管气相色谱（GC）分离，并在选择性离子监测采集模式（SIM）下通过质谱（MS）进行鉴定。在浓度为1000 ng/ml-1时，可待因在尿液中的回收率为72%，吗啡为80%。在所研究的范围内（0-1000 ng/ml-1），该方法对可待因和吗啡呈线性关系。