采用毛细管气相色谱法和质谱法同时鉴定和定量尿液中的可待因和吗啡。

Simultaneous identification and quantitation of codeine and morphine in urine by capillary gas chromatography and mass spectroscopy.

作者信息

Paul B D, Mell L D, Mitchell J M, Irving J, Novak A J

出版信息

J Anal Toxicol. 1985 Sep-Oct;9(5):222-6. doi: 10.1093/jat/9.5.222.

DOI:10.1093/jat/9.5.222

Abstract

An analytical procedure for simultaneous determination of codeine and morphine in urine is described. The detection of codeine and morphine is based on liquid-liquid extraction and derivatization to the acetylated compounds. The acetylated codeine and morphine are separated by capillary gas chromatography and identified mass spectrometrically by selected ion monitoring (SIM). Quantitative determination was carried out by SIM using nalorphine as internal standard. Excellent linearity was obtained over a concentration range of 25 to 800 ng/mL. The overall recovery for codeine and morphine in the extraction was found to be 58% and 40%, respectively. The on-column sensitivity for both compounds was 2 ng at a peak-to-noise ratio of 5:1. The derivatives, acetylcodeine, diacetylmorphine, and diacetylnalorphine were stable at room temperature for 72 hr.

摘要

本文描述了一种同时测定尿液中可待因和吗啡的分析方法。可待因和吗啡的检测基于液-液萃取并衍生化为乙酰化化合物。乙酰化的可待因和吗啡通过毛细管气相色谱分离，并通过选择离子监测（SIM）进行质谱鉴定。采用烯丙吗啡作为内标，通过SIM进行定量测定。在25至800 ng/mL的浓度范围内获得了良好的线性关系。萃取过程中可待因和吗啡的总回收率分别为58%和40%。两种化合物的柱上灵敏度在峰噪比为5:1时为2 ng。衍生物乙酰可待因、二乙酰吗啡和二乙酰烯丙吗啡在室温下72小时内稳定。