Kolosha V O, Fodor I
Department of Molecular Biology, USSR Academy of Sciences, Puschino.
Plant Mol Biol. 1990 Feb;14(2):147-61. doi: 10.1007/BF00018556.
The complete nucleotide sequence of Citrus limon 26S rDNA has been determined. The sequence has been aligned with large ribosomal RNA (L-rRNA) sequences of Escherichia coli, Saccharomyces cerevisiae and Oryza sativa. Nine extensive expansion segments in dicot 26S rRNA relative to E. coli 23S rRNA have been identified and compared with analogous segments of monocot, yeast, amphibian and human L-rRNAs. A secondary structure model for lemon 26S rRNA has been derived based on the refined model of E. coli 23S rRNA. It has been compared with other eukaryotic L-rRNAs models in terms of location of functionally important regions. Origin and evolution of L-rRNA expansion segments are discussed.
已经测定了柠檬（Citrus limon）26S rDNA的完整核苷酸序列。该序列已与大肠杆菌（Escherichia coli）、酿酒酵母（Saccharomyces cerevisiae）和水稻（Oryza sativa）的大核糖体RNA（L-rRNA）序列进行了比对。已鉴定出双子叶植物26S rRNA相对于大肠杆菌23S rRNA的九个广泛扩展区段，并与单子叶植物、酵母、两栖动物和人类L-rRNA的类似区段进行了比较。基于大肠杆菌23S rRNA的精细模型推导了柠檬26S rRNA的二级结构模型。已在功能重要区域的位置方面将其与其他真核L-rRNA模型进行了比较。讨论了L-rRNA扩展区段的起源和进化。
