Richardson L S, Belshe R B, Sly D L, London W T, Prevar D A, Camargo E, Chanock R M
J Med Virol. 1978;2(1):45-59. doi: 10.1002/jmv.1890020108.
Into 14 juvenile cebus monkeys that lacked serum antibodies for RS virus 10(8) plaque-forming units (pfu) of wild-type respiratory syncytial (RS) virus were inoculated transtracheally. Roentgenographic evidence of pneumonia developed in 13 of 14 infected animals. Gross pathologic changes occurred in each of the 13 monkeys that were sacrificed. Patchy areas of red consolidation were seen in the lower lobes 24 hours after inoculation, and there was progression to gray consolidation seven days later. Each of the infected animals had histologic evidence of interstitial pneumonia. Changes were detected in the lung as early as 24 hours after inoculation; they consisted primarily of infiltration of the alveolar wall. By the fourth to sixth day after inoculation there was marked interstitial thickening, pulmonary consolidation, formation of multinucleated giant cells and development of eosinophilic cytoplasmic inclusion bodies within alveolar cells. RS viral antigens, detected by indirect immunofluorescence, were distributed throughout cells of the alveolar wall and the bronchiolar epithelium. The virus grew to highest titer in the lungs on the fourth to sixth day after inoculation; up to 10(8) pfu/gram of tissue were detected. The cebus monkey represents the first experimental host to develop extensive pulmonary lesions during infection with respiratory syncytial virus.
对14只缺乏呼吸道合胞病毒血清抗体的幼年僧帽猴经气管接种10⁸蚀斑形成单位（pfu）的野生型呼吸道合胞（RS）病毒。14只受感染动物中有13只出现了肺炎的X线证据。对13只被处死的猴子均出现了大体病理变化。接种后24小时在下叶可见散在的红色实变区，7天后进展为灰色实变。每只受感染动物都有间质性肺炎的组织学证据。接种后24小时内最早在肺内检测到变化；主要表现为肺泡壁浸润。接种后第4至6天出现明显的间质增厚、肺实变、多核巨细胞形成以及肺泡细胞内嗜酸性细胞质包涵体形成。通过间接免疫荧光检测到的RS病毒抗原分布于肺泡壁和细支气管上皮的所有细胞。接种后第4至6天病毒在肺内达到最高滴度；每克组织检测到高达10⁸ pfu。僧帽猴是呼吸道合胞病毒感染期间出现广泛肺部病变的首个实验宿主。
