Laxalt A M, Cassia R O, Sanllorenti P M, Madrid E A, Andreu A B, Daleo G R, Conde R D, Lamattina L
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Argentina.
Plant Mol Biol. 1996 Mar;30(5):961-72. doi: 10.1007/BF00020807.
Plants respond to pathogen infection and environmental stress by regulating the coordinate expression of many stress-related genes. In plants, the expression of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is induced under environmental stress. This work was aimed at investigating whither the expression pattern of cytosolic GAPDH is also modulated upon infection of potato plants (Solanum tuberosum L.) with the late blight fungal agent Phytophthora infestans. Northern blot analysis showed the accumulation of the GAPDH gene transcripts in leaves and stems of inoculated potato plants. When tuber discs were treated with eicosapentaenoic acid (EPA), an elicitor found in P. infestans, GAPDH gene transcripts level increased. The increase was parallel to that of the hydroxymethyl glutharyl coenzyme A reductase (HMGR), an enzyme involved in pathogen defense reactions. Glucans obtained from P. infestans cell wall acts synergistically with EPA on GAPDH and HMGR gene induction. Salicylic acid, an endogenous signal for inducing systemic acquired resistance, was also effective in stimulating the GAPDH transcript accumulation in potato leaves. These experiments suggest that related multi-component factors, which are part of both primary and secondary metabolism, are probably regulated by similar signal transduction pathways when they are induced under biotic or abiotic stress conditions.
植物通过调节许多与胁迫相关基因的协同表达来应对病原体感染和环境胁迫。在植物中，糖酵解酶甘油醛-3-磷酸脱氢酶（GAPDH）的表达在环境胁迫下被诱导。这项工作旨在研究马铃薯（Solanum tuberosum L.）感染晚疫病菌（Phytophthora infestans）后，胞质GAPDH的表达模式是否也受到调节。Northern印迹分析表明，接种马铃薯植株的叶片和茎中存在GAPDH基因转录本的积累。当用二十碳五烯酸（EPA，一种在致病疫霉中发现的激发子）处理块茎切片时，GAPDH基因转录本水平增加。这种增加与参与病原体防御反应的羟甲基戊二酰辅酶A还原酶（HMGR）的增加平行。从致病疫霉细胞壁获得的葡聚糖与EPA协同作用，诱导GAPDH和HMGR基因表达。水杨酸是诱导系统获得性抗性的内源性信号，它也能有效刺激马铃薯叶片中GAPDH转录本的积累。这些实验表明，作为初级和次级代谢一部分的相关多组分因子，在生物或非生物胁迫条件下被诱导时，可能受相似的信号转导途径调控。
