Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, United States.
Anal Chem. 2010 Nov 15;82(22):9344-9. doi: 10.1021/ac1019123. Epub 2010 Oct 28.
Inefficient ionization and poor transmission of the charged species produced by an electrospray from the ambient pressure mass spectrometer source into the high vacuum region required for mass analysis significantly limits achievable sensitivity. Here, we present evidence that, when operated at flow rates of 50 nL/min, a new electrospray-based ion source operated at ∼20 Torr can deliver ∼50% of the analyte ions initially in the solution as charged desolvated species into the rough vacuum region of mass spectrometers. The ion source can be tuned to optimize the analyte signal for readily ionized species while reducing the background contribution.
在大气压质谱源中，电喷雾产生的带电物质的离子化效率低，传输效率差，无法进入质量分析所需的高真空区域，这极大地限制了可达到的灵敏度。在这里，我们提供的证据表明，当以 50nL/min 的流速运行时，一种新的基于电喷雾的离子源在约 20 托下运行，可以将初始溶液中约 50%的分析物离子以带电去溶剂化的形式输送到质谱仪的粗真空区域。可以调整离子源以优化易电离物质的分析物信号，同时减少背景贡献。
