• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

植物线粒体开放阅读框orf221编码一种膜结合蛋白。

The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein.

作者信息

Prioli L M, Huang J, Levings C S

机构信息

Departamento de Genética e Evolução, Universidade Estadual de Campinas (UNICAMP), SP, Brasil.

出版信息

Plant Mol Biol. 1993 Oct;23(2):287-95. doi: 10.1007/BF00029005.

DOI:10.1007/BF00029005
PMID:8219065
Abstract

We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.

摘要

我们已经证明，开放阅读框orf221是一个活跃的线粒体基因，它编码一种新型线粒体多肽。orf221序列在高等植物中很常见，但在动物和真菌线粒体中不存在。用针对ORF221融合蛋白产生的多克隆抗体检测到一种表观分子量为21,000的线粒体多肽。在离体翻译后用抗ORF221抗体进行免疫沉淀表明，这种多肽由植物线粒体中的orf221基因编码。在玉米（Zea mays L.）的几个自交系以及其他植物物种的正常（N）、cms-T和cms-C细胞质中，ORF221被发现是一种线粒体膜蛋白。

相似文献

1
The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein.植物线粒体开放阅读框orf221编码一种膜结合蛋白。
Plant Mol Biol. 1993 Oct;23(2):287-95. doi: 10.1007/BF00029005.
2
The protein-encoding gene T-urf13 is not edited in maize mitochondria.蛋白质编码基因T-urf13在玉米线粒体中不发生编辑。
Plant Mol Biol. 1991 Nov;17(5):1083-8. doi: 10.1007/BF00037148.
3
The nuclear gene Rf3 affects the expression of the mitochondrial chimeric sequence R implicated in S-type male sterility in maize.核基因Rf3影响与玉米S型雄性不育相关的线粒体嵌合序列R的表达。
Genetics. 1997 Oct;147(2):847-60. doi: 10.1093/genetics/147.2.847.
4
Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria.玉米和烟草线粒体中urf13-T基因及一个未鉴定阅读框ORF 25的特征分析
Curr Genet. 1987;12(6):457-63. doi: 10.1007/BF00434824.
5
Integrated R2 sequence in mitochondria of fertile B37N maize encodes and expresses a 130 kD polypeptide similar to that encoded by the S2 episome of S-type male sterile plants.可育的B37N玉米线粒体中的整合R2序列编码并表达一种130kD的多肽，该多肽与S型雄性不育植株的S2附加体所编码的多肽相似。
Nucleic Acids Res. 1989 Jan 11;17(1):405-22. doi: 10.1093/nar/17.1.405.
6
Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids.小仓细胞质雄性不育（CMS）相关的orf138在雄性不育的芸苔属体细胞杂种中被翻译成一种线粒体膜多肽。
Mol Gen Genet. 1994 Jun 3;243(5):540-7. doi: 10.1007/BF00284202.
7
The CMS-associated 16 kDa protein encoded by orfH522 in the PET1 cytoplasm is also present in other male-sterile cytoplasms of sunflower.PET1细胞质中由orfH522编码的与CMS相关的16 kDa蛋白也存在于向日葵的其他雄性不育细胞质中。
Plant Mol Biol. 1996 Feb;30(3):523-38. doi: 10.1007/BF00049329.
8
Interaction of nuclear and mitochondrial genomes in the alteration of maize mitochondrial orf221 transcripts.核基因组与线粒体基因组在改变玉米线粒体 orf221 转录本中的相互作用。
Theor Appl Genet. 1994 Dec;89(7-8):951-8. doi: 10.1007/BF00224523.
9
Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum.重组与玉米和高粱线粒体基因组的多态性相关。
Philos Trans R Soc Lond B Biol Sci. 1988 May 31;319(1193):187-98. doi: 10.1098/rstb.1988.0042.
10
Plant mitochondrial mutations and male sterility.植物线粒体突变与雄性不育
Annu Rev Genet. 1991;25:461-86. doi: 10.1146/annurev.ge.25.120191.002333.

引用本文的文献

1
Interaction of nuclear and mitochondrial genomes in the alteration of maize mitochondrial orf221 transcripts.核基因组与线粒体基因组在改变玉米线粒体 orf221 转录本中的相互作用。
Theor Appl Genet. 1994 Dec;89(7-8):951-8. doi: 10.1007/BF00224523.
2
Interactions of mitochondrial and nuclear genes that affect male gametophyte development.影响雄配子体发育的线粒体基因与核基因的相互作用。
Plant Cell. 2004;16 Suppl(Suppl):S154-69. doi: 10.1105/tpc.015966. Epub 2004 May 6.
3
The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b.

本文引用的文献

1
Higher plant mitochondrial DNA expression : 1. Variant expression of the plant mitochondrial open reading frame, ORF25, in B37N and B73N maize lines.高等植物线粒体 DNA 的表达：1. 在 B37N 和 B73N 玉米品系中线粒体开放阅读框 ORF25 的可变表达。
Theor Appl Genet. 1991 Oct;82(6):765-70. doi: 10.1007/BF00227323.
2
Higher plant mitochondrial DNA expression : 2. Influence of nuclear background on the transcription of a mitochondrial open reading frame, ORF25.高等植物线粒体 DNA 表达：2. 核背景对线粒体开放阅读框 ORF25 转录的影响。
Theor Appl Genet. 1991 Oct;82(6):723-8. doi: 10.1007/BF00227317.
3
The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility.
神秘的线粒体开放阅读框ymf39编码ATP合酶链b。
Nucleic Acids Res. 2003 May 1;31(9):2353-60. doi: 10.1093/nar/gkg326.
4
Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green algae.紫菜线粒体DNA的完整序列。蓝藻内含子以及红藻和绿藻的共同祖先。
Plant Cell. 1999 Sep;11(9):1675-94. doi: 10.1105/tpc.11.9.1675.
5
Genome structure and gene content in protist mitochondrial DNAs.原生生物线粒体DNA的基因组结构与基因含量
Nucleic Acids Res. 1998 Feb 15;26(4):865-78. doi: 10.1093/nar/26.4.865.
6
Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm.高粱线粒体orf25与雄性不育细胞质的相关嵌合结构。
Curr Genet. 1996 Feb;29(3):265-74. doi: 10.1007/BF02221557.
玉米的得克萨斯细胞质：细胞质雄性不育性和易感性。
Science. 1990 Nov 16;250(4983):942-7. doi: 10.1126/science.250.4983.942.
4
A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize.与玉米 T 细胞质雄性不育相关的线粒体蛋白。
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5374-8. doi: 10.1073/pnas.84.15.5374.
5
Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame.德克萨斯州（T）细胞质玉米中的雄性育性和毒素不敏感性突变与线粒体开放阅读框的移码有关。
Proc Natl Acad Sci U S A. 1987 May;84(9):2858-62. doi: 10.1073/pnas.84.9.2858.
6
The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site.玉米线粒体中的细胞色素 b 基因不含内含子，且其上游存在一个潜在的核糖体结合位点。
EMBO J. 1984 Sep;3(9):2107-13. doi: 10.1002/j.1460-2075.1984.tb02098.x.
7
Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes.大鼠肝脏过氧化物酶体膜的多肽和磷脂组成：与内质网和线粒体膜的比较。
J Cell Biol. 1982 Apr;93(1):103-10. doi: 10.1083/jcb.93.1.103.
8
Protein synthesis by isolated plant mitochondria.离体植物线粒体的蛋白质合成
Methods Enzymol. 1983;97:476-84. doi: 10.1016/0076-6879(83)97156-2.
9
Immunoprecipitation of proteins from cell-free translations.从无细胞翻译体系中进行蛋白质免疫沉淀。
Methods Enzymol. 1983;96:111-20. doi: 10.1016/s0076-6879(83)96012-3.
10
Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll.通过在Percoll密度梯度中进行等密度离心法纯化植物线粒体。
Arch Biochem Biophys. 1982 Aug;217(1):312-23. doi: 10.1016/0003-9861(82)90507-0.