Acar B, Simsek G Güler, Oguztuzun S, Zaim M, Karasen R Murat
Department of Otorhinolaryngology, Kecioren Training and Research Hospital, Ankara, Turkey.
J Laryngol Otol. 2011 Mar;125(3):227-30. doi: 10.1017/S0022215110002161. Epub 2010 Oct 29.
This study aimed to demonstrate the histological and immunohistological features of skin biopsy specimens from patients complaining of isolated itching of the external auditory canal.
A prospective, case-control study was performed of 24 patients undergoing evaluation for contact dermatitis of the external auditory canal, and 24 controls. Skin biopsies were examined histologically by a single, blinded dermatopathologist, using light microscopy, to determine histopathological characteristics. The immunohistological presence of the antimicrobial peptides human β-defensin-3 and LL-37 cathelicidin was also assessed. Findings for patients and controls were compared.
There was a statistically significant difference in the degree of inflammation, comparing patients and controls (p < 0.05). There was no significant difference in the presence of spongiotic changes, comparing patients and controls (p > 0.05). Furthermore, the patients' skin biopsies did not show pronounced expression of human β-defensin-3 or LL-37 cathelicidin.
Histological and immunohistological examination of skin biopsies from cases of isolated itching of the external auditory canal did not support a diagnosis of dermatitis.
本研究旨在阐明主诉外耳道单纯瘙痒患者皮肤活检标本的组织学和免疫组织学特征。
对24例接受外耳道接触性皮炎评估的患者及24例对照进行前瞻性病例对照研究。由一名盲法皮肤科病理学家使用光学显微镜对皮肤活检标本进行组织学检查，以确定组织病理学特征。还评估了抗菌肽人β-防御素-3和LL-37 杀菌肽的免疫组织学存在情况。比较患者和对照的结果。
比较患者和对照，炎症程度存在统计学显著差异（p < 0.05）。比较患者和对照，海绵状改变的存在无显著差异（p > 0.05）。此外，患者的皮肤活检未显示人β-防御素-3或LL-37杀菌肽的明显表达。
外耳道单纯瘙痒病例皮肤活检的组织学和免疫组织学检查不支持皮炎的诊断。
