Luo Chun-fang, Hong Hui-li, Lu Yu-lan, Wang Hui, Liu Ming-qiu
Department of Obstetrics and Gynecology, The Third Hospital of Wuhan, Wuhan 430060, China.
Zhonghua Zhong Liu Za Zhi. 2010 Jul;32(7):533-8.
To investigate the expressions of bFGF and PTEN in cervical carcinoma and their clinical significance.
Tissue microarray technique and immunohistochemistry SP method were used to detect the expressions of bFGF and PTEN in 143 cases of invasive carcinoma of cervix (ICC) and 20 cases of normal cervical epithelium remote from tumor (NCE). The relationship between the expressions of bFGF and PTEN in ICC and some factors relating to clinical pathology of cervical carcinoma such as histopathological grading, lymph node metastasis, stroma involvement and FIGO staging were analyzed.
The rate of the positive expression of bFGF in ICC was significantly higher than that in NCE 88.8% (127/143) vs. 25.0% (5/20, P = 0.000). The rate of positive expression of PTEN in ICC was significantly lower than that in NCE 67.1% (96/143) vs. 100.0% (20/20, P = 0.000). The expression of bFGF was positively correlated with lymph node metastasis and histopathological grading (r = 0.239, P = 0.004 and r = 0.369, P = 0.000, respectively). The expression of PTEN was negatively correlated with FIGO staging, histopathological grading and lymph node metastasis (r = -0.189, P = 0.024; r = -0.211, P = 0.011; r = -0.321, P = 0.000, respectively). The expression of bFGF was negatively correlated with the expression of PTEN in ICC (r = -0.261, P = 0.002).
The overexpression of bFGF and underexpression of PTEN are closely related to the invasion and growth of cervical carcinoma. Detection of the expression of both bFGF and PTEN may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.
探讨碱性成纤维细胞生长因子（bFGF）和第10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）在宫颈癌中的表达及其临床意义。
采用组织芯片技术和免疫组织化学SP法检测143例宫颈浸润癌（ICC）及20例距肿瘤较远的正常宫颈上皮（NCE）中bFGF和PTEN的表达。分析ICC中bFGF和PTEN的表达与宫颈癌临床病理相关因素如组织病理学分级、淋巴结转移、间质浸润及国际妇产科联盟（FIGO）分期之间的关系。
ICC中bFGF阳性表达率显著高于NCE，分别为88.8%（127/143）和25.0%（5/20，P = 0.000）。ICC中PTEN阳性表达率显著低于NCE，分别为67.1%（96/143）和100.0%（20/20，P = 0.000）。bFGF的表达与淋巴结转移及组织病理学分级呈正相关（r = 0.239，P = 0.004；r = 0.369，P = 0.000）。PTEN的表达与FIGO分期、组织病理学分级及淋巴结转移呈负相关（r = -0.189，P = 0.024；r = -0.211，P = 0.011；r = -0.321，P = 0.000）。ICC中bFGF的表达与PTEN的表达呈负相关（r = -0.261，P = 0.002）。
bFGF的过表达和PTEN的低表达与宫颈癌的侵袭和生长密切相关。检测bFGF和PTEN的表达可能有助于进一步了解宫颈癌的生物学行为并预测其预后。
