Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical College, Asahikawa, Hokkaido 078-8510, Japan.
J Clin Gastroenterol. 2011 Jul;45(6):507-13. doi: 10.1097/MCG.0b013e3181fbe22a.
A method for the differential diagnosis of intestinal lymphomas resembling lymphoid hyperplasia (LH) by endoscopy remains to be clearly established.
To evaluate the usefulness of autofluorescence imaging (AFI) in diagnosing intestinal lymphoma.
Single-center study.
Prospective study.
One hundred forty-three samples obtained from the intestinal tissues of 21 patients with malignant lymphoma were included in the study.
The terminal ileum and entire colon were observed using conventional endoscopy equipped with AFI. The AFI images were taken by 3 endoscopists and then were evaluated by 3 predominant color intensities; green, magenta, and blended. To quantify the strength of fluorescence captured by AFI, the area of the obtained biopsy specimens on images was manually traced, the signal density of either magenta or green was measured, and then the ratio of the reverse gamma value of green divided by that of magenta was defined as the Fluorescence index (F index).
The ability to use AFI to distinguish intestinal lymphoma from normal or LH.
The cell density is inversely proportional to the F index. The F index of lymphoma was significantly lower than that of normal mucosa or LH. The visual classification of AFI showed the overall accuracy in diagnosing lymphoma was 91.5%, and was well correlated with the F index.
Single-center study.
AFI-embossed lymphoma lesions seemed as magenta and could be discriminated from LH or normal mucosa with a high overall accuracy through perception of the cell density of the lesion. Therefore, AFI is considered to be an effective procedure for determining the accurate stage and appropriate therapy in intestinal lymphoma.
内镜下鉴别类似于淋巴组织增生（LH）的肠道淋巴瘤的方法仍需明确建立。
评估自体荧光成像（AFI）在诊断肠道淋巴瘤中的作用。
单中心研究。
前瞻性研究。
本研究纳入 21 例恶性淋巴瘤患者的 143 个肠道组织样本。
使用配备 AFI 的常规内镜观察末端回肠和整个结肠。由 3 名内镜医师拍摄 AFI 图像，然后通过 3 种主要颜色强度（绿色、品红色和混合色）进行评估。为了量化 AFI 捕获的荧光强度，手动跟踪图像上获得的活检标本的面积，测量品红色或绿色的信号密度，然后定义绿色与品红色的反向伽马值之比为荧光指数（F 指数）。
使用 AFI 区分肠道淋巴瘤与正常或 LH 的能力。
细胞密度与 F 指数成反比。淋巴瘤的 F 指数明显低于正常黏膜或 LH。AFI 的视觉分类显示，诊断淋巴瘤的总体准确率为 91.5%，与 F 指数相关性良好。
单中心研究。
AFI 压痕样的淋巴瘤病变呈品红色，通过感知病变细胞密度，可以与 LH 或正常黏膜区分，具有较高的总体准确率。因此，AFI 被认为是一种确定肠道淋巴瘤准确分期和适当治疗的有效方法。
