日本正常眼压性青光眼患者的HLA - DRB1和HLA - DQB1等位基因分型
Genotyping HLA-DRB1 and HLA-DQB1 alleles in Japanese patients with normal tension glaucoma.
作者信息
Suzuki Misa, Meguro Akira, Ota Masao, Nomura Eiichi, Kato Tetsuo, Nomura Naoko, Kashiwagi Kenji, Mabuchi Fumihiko, Iijima Hiroyuki, Kawase Kazuhide, Yamamoto Tetsuya, Nakamura Makoto, Negi Akira, Sagara Takeshi, Nishida Teruo, Inatani Masaru, Tanihara Hidenobu, Aihara Makoto, Araie Makoto, Fukuchi Takeo, Abe Haruki, Higashide Tomomi, Sugiyama Kazuhisa, Kanamoto Takashi, Kiuchi Yoshiaki, Iwase Aiko, Ohno Shigeaki, Inoko Hidetoshi, Mizuki Nobuhisa
机构信息
Department of Ophthalmology and Visual Science Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
出版信息
Mol Vis. 2010 Sep 15;16:1874-9.
PURPOSE
Normal tension glaucoma (NTG) is a subtype of glaucoma in which intraocular pressure is within the statistically normal range. NTG may be associated with an immune disorder. The aim of this study was to determine whether specific alleles in the human leukocyte antigen (HLA)-DRB1 and HLA-DQB1 genes correlated with NTG in Japanese patients.
METHODS
We genotyped the HLA-DRB1 and HLA-DQB1 alleles in 113 Japanese patients with NTG and in 184 healthy Japanese control subjects using the polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) Luminex method. We assessed the allelic diversity in patients and controls.
RESULTS
There were no statistically significant differences in the allele frequency of HLADRB1 and HLA-DQB1 between NTG patients and control subjects, and no HLA-DRB1-HLA-DQB1 haplotypes demonstrated any significant association with NTG.
CONCLUSIONS
Our findings suggest that HLA-DRB1 and HLA-DQB1 polymorphisms have no significant effect on the development of NTG in Japanese patients.
目的
正常眼压性青光眼（NTG）是青光眼的一种亚型，其眼压在统计学正常范围内。NTG可能与免疫紊乱有关。本研究的目的是确定人类白细胞抗原（HLA）-DRB1和HLA-DQB1基因中的特定等位基因是否与日本患者的NTG相关。
方法
我们使用聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）Luminex方法对113例日本NTG患者和184例健康日本对照者的HLA-DRB1和HLA-DQB1等位基因进行基因分型。我们评估了患者和对照者的等位基因多样性。
结果
NTG患者与对照者之间HLA-DRB1和HLA-DQB1的等位基因频率无统计学显著差异，且没有HLA-DRB1-HLA-DQB1单倍型显示与NTG有任何显著关联。
结论
我们的研究结果表明，HLA-DRB1和HLA-DQB1多态性对日本患者NTG的发生没有显著影响。
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