Metabonomics and Multidisciplinary Laboratory for Trauma Research, Trauma Surgery, East Branch of Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610101, China.
Chin Med J (Engl). 2010 Sep;123(17):2315-9.
Spinal cord injury (SCI) is a complex trauma that consists of multiple pathological mechanisms involving cytotoxic, oxidation stress and immune-endocrine. This study aimed to establish plasma metabonomics fingerprinting atlas for SCI using (1)H nuclear magnetic resonance (NMR) based metabonomics methodology and principal component analysis techniques.
Nine Sprague-Dawley (SD) male rats were randomly divided into SCI, normal and sham-operation control groups. Plasma samples were collected for (1)H NMR spectroscopy 3 days after operation. The NMR data were analyzed using principal component analysis technique with Matlab software.
Metabonomics analysis was able to distinguish the three groups (SCI, normal control, sham-operation). The fingerprinting atlas indicated that, compared with those without SCI, the SCI group demonstrated the following characteristics with regard to second principal component: it is made up of fatty acids, myc-inositol, arginine, very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), triglyceride (TG), glucose, and 3-methyl-histamine.
The data indicated that SCI results in several significant changes in plasma metabolism early on and that a metabonomics approach based on (1)H NMR spectroscopy can provide a metabolic profile comprising several metabolite classes and allow for relative quantification of such changes. The results also provided support for further development and application of metabonomics technologies for studying SCI and for the utilization of multivariate models for classifying the extent of trauma within an individual.
脊髓损伤（SCI）是一种复杂的创伤，包含涉及细胞毒性、氧化应激和免疫内分泌的多种病理机制。本研究旨在使用基于（1）H 核磁共振（NMR）的代谢组学方法和主成分分析技术，为 SCI 建立血浆代谢组学指纹图谱。
9 只雄性 Sprague-Dawley（SD）大鼠随机分为 SCI、正常和假手术对照组。术后 3 天采集血浆样本进行（1）H NMR 光谱分析。使用 Matlab 软件的主成分分析技术对 NMR 数据进行分析。
代谢组学分析能够区分三组（SCI、正常对照组、假手术组）。指纹图谱表明，与未发生 SCI 的大鼠相比，SCI 组在第二主成分上由以下特征组成：脂肪酸、肌醇、精氨酸、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、甘油三酯（TG）、葡萄糖和 3-甲基组氨酸。
数据表明，SCI 导致早期血浆代谢发生了多种显著变化，基于（1）H NMR 光谱的代谢组学方法可以提供包含多个代谢物类别的代谢谱，并允许对这些变化进行相对定量。结果还为代谢组学技术在 SCI 研究中的进一步发展和应用提供了支持，并为利用多元模型对个体创伤程度进行分类提供了支持。
