Medical Faculty, Department of Obstetrics and Gynecology, Kahramanmaras Sutcuimam University, Kahramanmaras, Turkey.
J Pediatr Surg. 2010 Nov;45(11):2203-9. doi: 10.1016/j.jpedsurg.2010.07.011.
BACKGROUND/PURPOSE: The aim of the study was to evaluate the effects of tadalafil (TDF) on ischemia/reperfusion (I/R) injury in rat ovaries.
Thirty-five female Sprague-Dawley rats were randomly divided into 5 groups (n = 7): sham (S), I/R1, I/R2, TDF1, and TDF2. In the I/R1 and TDF1 groups, 3-hour ischemia was followed by 12-hour reperfusion; and in the I/R2 and TDF2 groups, 3-hour ischemia was followed by 24-hour reperfusion. In the TDF groups, 30 minutes before reperfusion, a single dose of 5 mg/kg TDF was administered intraperitoneally. The ovarian tissue levels of malondialdehyde and nitric oxide (NO), and the activities of superoxide dismutase and catalase were measured biochemically. Tissue damage to ovarian tissue was scored by histopathologic examination.
The tissue malondialdehyde levels were significantly higher and the catalase and superoxide dismutase activities were significantly lower in the I/R groups compared with the S and TDF groups (P < .05). The NO levels were significantly higher in the TDF1 group than the S and I/R1 groups (P < .05). Although the NO levels were increased in the TDF2 group compared with the I/R2 group, the difference was not significant. Ovarian tissue damage scores of the I/R groups were significantly higher than those of the S group (P < .05). Treatment with TDF significantly decreased the ovarian tissue damage scores in the TDF groups compared with the I/R groups (P < .05).
Tadalafil is effective in preventing tissue damage induced by I/R in rat ovaries.
背景/目的：本研究旨在评估他达拉非（TDF）对大鼠卵巢缺血/再灌注（I/R）损伤的影响。
35 只雌性 Sprague-Dawley 大鼠随机分为 5 组（n = 7）：假手术（S）组、I/R1 组、I/R2 组、TDF1 组和 TDF2 组。在 I/R1 和 TDF1 组中，进行 3 小时的缺血，随后进行 12 小时的再灌注；在 I/R2 和 TDF2 组中，进行 3 小时的缺血，随后进行 24 小时的再灌注。在 TDF 组中，在再灌注前 30 分钟，给予单次剂量 5mg/kg TDF 腹膜内注射。通过生化方法测量卵巢组织中丙二醛和一氧化氮（NO）的水平，以及超氧化物歧化酶和过氧化氢酶的活性。通过组织病理学检查对卵巢组织损伤进行评分。
与 S 组和 TDF 组相比，I/R 组的组织丙二醛水平显著升高，过氧化氢酶和超氧化物歧化酶的活性显著降低（P <.05）。TDF1 组的 NO 水平显著高于 S 组和 I/R1 组（P <.05）。虽然 TDF2 组的 NO 水平与 I/R2 组相比有所升高，但差异无统计学意义。I/R 组的卵巢组织损伤评分明显高于 S 组（P <.05）。与 I/R 组相比，TDF 治疗显著降低了 TDF 组的卵巢组织损伤评分（P <.05）。
他达拉非可有效预防大鼠卵巢 I/R 诱导的组织损伤。
