Special Pathogens Laboratory, Pittsburgh, PA, USA.
Am J Infect Control. 2010 Nov;38(9):678-82. doi: 10.1016/j.ajic.2010.07.002.
The Centers for Disease Control and Prevention's guidelines for hand hygiene state that the use of alcohol-based hand wipes is not an effective substitute for the use of an alcohol-based hand rub or handwashing with an antimicrobial soap and water. The objective of this study was to determine whether a hand wipe with higher ethanol content (65.9%) is as effective as an ethanol hand rub or antimicrobial soap in removing bacteria and spores from hands.
In two separate experiments, the hands of 7 subjects were inoculated with a suspension of Serratia marcescens or Geobacillus stearothermophilus. Subjects washed with each of 3 different products: 65.9% ethanol hand wipes (Sani-Hands ALC), 62% ethanol gel rub (Purell), and antimicrobial soap containing 0.75% triclosan (Kindest Kare).
A total of 56 observations were analyzed for S marcescens removal and 70 observations were analyzed for G stearothermophilus removal. The rank order of product efficacy for both bacteria and spore removal was antibacterial soap > 65.9% ethanol hand wipes >62% ethanol hand rub. Mean S marcescens log reductions (±SD) for the 65.9% ethanol alcohol wipe, 62% ethanol alcohol rub, and antimicrobial foam soap were 3.44 ± 0.847, 2.32 ± 1.065, and 4.44 ± 1.018, respectively (P < .001). Mean G stearothermophilus log reductions for the 65.9% ethanol wipe, 62% ethanol rub, and antimicrobial foam soap were 0.51 ± 0.26, -0.8 ± 0.32 increase over baseline, and 1.72 ± 0.62, respectively (P < .001).
The alcohol-based hand wipe containing 65.9% ethanol was significantly more effective than the 62% ethanol rub in reducing the number of viable bacteria and spores on the hands.
疾病控制与预防中心的手部卫生指南指出，使用酒精擦手巾不是替代使用酒精搓手液或用抗菌肥皂和水洗手的有效方法。本研究的目的是确定含有更高乙醇含量（65.9%）的擦手巾是否与乙醇搓手液或抗菌肥皂一样有效，可从手上清除细菌和孢子。
在两项独立的实验中，7 名受试者的手被接种了马氏肠杆菌或嗜热脂肪芽孢杆菌的悬浮液。受试者用 3 种不同产品中的每一种进行清洗：65.9%乙醇擦手巾（Sani-Hands ALC）、62%乙醇凝胶搓手液（Purell）和含有 0.75%三氯生的抗菌肥皂（Kindest Kare）。
共分析了 56 次对马氏肠杆菌清除的观察结果和 70 次对嗜热脂肪芽孢杆菌清除的观察结果。这两种细菌和孢子清除的产品效果排序是抗菌肥皂>65.9%乙醇擦手巾>62%乙醇手搓液。65.9%乙醇酒精擦手巾、62%乙醇酒精搓手液和抗菌泡沫皂对马氏肠杆菌的平均 log 减少量（±SD）分别为 3.44 ± 0.847、2.32 ± 1.065 和 4.44 ± 1.018（P<.001）。65.9%乙醇擦手巾、62%乙醇搓手液和抗菌泡沫皂对嗜热脂肪芽孢杆菌的平均 log 减少量分别为 0.51 ± 0.26、基线水平增加 0.8 ± 0.32 和 1.72 ± 0.62（P<.001）。
含 65.9%乙醇的酒精擦手巾在减少手上存活细菌和孢子数量方面明显比 62%乙醇搓手液更有效。
