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牙龈非反应性和反应性病变中肥大细胞的定量分析：一项使用甲苯胺蓝和免疫组化标志物肥大细胞类胰蛋白酶的比较研究。

Quantification of mast cells in nonreactive and reactive lesions of gingiva: A comparative study using toluidine blue and immunohistochemical marker mast cell tryptase.

作者信息

Jain Amisha, Jaiswal Shradha, Vikey Ashok, Bagulkar Bhupesh, Bhat Atul, Shujalpurkar Anjali

机构信息

Department of Oral Pathology and Microbiology, Sri Aurobindo College of Dentistry, Indore, Madhya Pradesh, India.

Department of Oral Pathology and Microbiology, Government Dental College, Indore, Madhya Pradesh, India.

出版信息

J Oral Maxillofac Pathol. 2021 Sep-Dec;25(3):550-551. doi: 10.4103/jomfp.JOMFP_267_19. Epub 2022 Jan 11.

DOI:10.4103/jomfp.JOMFP_267_19
PMID:35281132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8859601/
Abstract

BACKGROUND

Mast cells (MCs) are immune cells derived from a multipotent CD34 precursor. The most significant identifying feature of MCs is the presence of metachromatic granules. MCs are increased in oral reactive lesions and are possibly involved in pathogenesis of these lesions.

OBJECTIVES

  1. To compare the number of MCs between reactive and nonreactive lesions of gingiva using toluidine blue (TB) and mast cell tryptase (MCT) as a specific marker for MCs 2. To compare the staining specificity/efficacy of TB and MCT.

METHODOLOGY

The study sample comprised 90 tissues which were divided into three groups: Group A comprised 30 cases of pyogenic granuloma (PG), Group B consisted of 30 cases of gingival hyperplasia (GH) and Group C comprised 30 cases of pericoronitis. Staining was done between 1% TB and immunohistochemistry (IHC) marker MCT.

RESULTS

A significant increase in number of MCs was observed in PG as compared to GH and pericoronitis. IHC marker MCT proved to be a more specific marker for MCs compared to TB.

CONCLUSION

IHC marker MCT is a specific marker compared to TB. The position of MCs changed from juxtaepithelial in GH to deeper connective tissue in PG which was in correlation with the proliferating tissue that is epithelium in GH and blood vessel in PG.

摘要

背景

肥大细胞（MCs）是源自多能CD34前体的免疫细胞。肥大细胞最显著的识别特征是存在异染性颗粒。肥大细胞在口腔反应性病变中增多，可能参与这些病变的发病机制。

目的

  1. 使用甲苯胺蓝（TB）和肥大细胞类胰蛋白酶（MCT）作为肥大细胞的特异性标志物，比较牙龈反应性病变和非反应性病变中肥大细胞的数量。2. 比较甲苯胺蓝和肥大细胞类胰蛋白酶的染色特异性/效果。

方法

研究样本包括90个组织，分为三组：A组包括30例化脓性肉芽肿（PG），B组由30例牙龈增生（GH）组成，C组包括30例冠周炎。采用1%甲苯胺蓝染色和免疫组织化学（IHC）标志物肥大细胞类胰蛋白酶进行染色。

结果

与牙龈增生和冠周炎相比，化脓性肉芽肿中肥大细胞数量显著增加。免疫组织化学标志物肥大细胞类胰蛋白酶被证明是比甲苯胺蓝更特异的肥大细胞标志物。

结论

与甲苯胺蓝相比，免疫组织化学标志物肥大细胞类胰蛋白酶是一种特异性标志物。肥大细胞的位置从牙龈增生中的近上皮位置变为化脓性肉芽肿中更深的结缔组织位置，这与增生组织相关，牙龈增生中的增生组织是上皮，化脓性肉芽肿中的增生组织是血管。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62ca/8859601/3a259b4e5c77/JOMFP-25-550-g011.jpg
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