Shojaei Setareh, Jamshidi Shokoofeh, Roshanaei Ghodratollah, Modabbernia Shirin, Farzin Bahman
Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran;
Dental Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran;
J Dent (Shiraz). 2015 Mar;16(1 Suppl):10-4.
Soft tissue reactive lesions are the most common lesions of the oral cavity. Although many studies have shown the interaction of mast cells with fibroblasts and their participation in fibrosis, the role of mast cells in these lesions is not well understood.
The aim of this study was to evaluate the mast cells (MCs) count in oral soft-tissue reactive lesions including peripheral giant cell granuloma (PGCG), peripheral ossifying fibroma (POF), irritation fibroma (IF) and normal oral mucosa.
In this cross-sectional study, 50 samples including IF, PGCG, POF (14 cases for each group) and 8 cases of normal oral mucosa were stained with tryptase antibody through immunohistochemistry. The number of mast cells was counted in 5HPF containing maximum counts for each section stained with tryptase. Statistical analysis including Chi-square test and Tukey test with a significance level of 0.05 were considered.
The number of MCs was found to have increased in reactive lesions compared with normal oral mucosa. MCs count in the POF group was higher than the others.
These findings suggest a possible role of mast cells in the pathogenesis of reactive oral lesions and induction of fibrous tissues. Chemical mediators released from mast cells might influence other cells, especially fibroblasts, to induce fibrosis.
软组织反应性病变是口腔中最常见的病变。尽管许多研究已经表明肥大细胞与成纤维细胞之间的相互作用以及它们在纤维化中的参与，但肥大细胞在这些病变中的作用尚未得到充分理解。
本研究的目的是评估口腔软组织反应性病变中的肥大细胞（MCs）计数，包括外周巨细胞肉芽肿（PGCG）、外周骨化性纤维瘤（POF）、刺激性纤维瘤（IF）和正常口腔黏膜。
在这项横断面研究中，通过免疫组织化学用色氨酸酶抗体对50个样本进行染色，这些样本包括IF、PGCG、POF（每组14例）和8例正常口腔黏膜。在色氨酸酶染色的每个切片的5个高倍视野（HPF）中计数肥大细胞的数量，这些视野包含最大细胞数。进行了包括卡方检验和Tukey检验在内的统计分析，显著性水平为0.05。
与正常口腔黏膜相比，发现反应性病变中的MCs数量增加。POF组中的MCs计数高于其他组。
这些发现表明肥大细胞在反应性口腔病变的发病机制和纤维组织诱导中可能发挥作用。肥大细胞释放的化学介质可能影响其他细胞，尤其是成纤维细胞，从而诱导纤维化。
