Photobiology Division, Indian Institute of Toxicology Research, (CSIR), MG Marg, Lucknow, India.
Bioresour Technol. 2011 Feb;102(3):2979-84. doi: 10.1016/j.biortech.2010.10.005. Epub 2010 Oct 8.
The metabolizing potential of a bacterial strain Rhodococcus MTCC 6716, isolated from the gut of an Indian earthworm (Metaphire posthuma) was studied for endosulfan bioremediation. In the present work, the optimum conditions for the maximum growth, kinetic of endosulfan degradation, regression equation, half life and correlation coefficient were studied. Endosulfan induced alterations in the expression of mRNA and protein of specific endosulfan metabolizing marker gene (Esd) was studied. Maximum growth of bacteria was observed at pH 7.0, 30°C and 0.085 M sodium chloride concentration in a liquid culture medium. Endosulfan was degraded by Rhodococcus strain up to 97.23% within 15 days without producing toxic metabolite and with strong correlation coefficient (-0.728) and half life 5.99 days. Endosulfan degradation was mediated through gene(s) present in genomic DNA. Expression of marker gene was found endosulfan concentration dependent. The results suggest that this novel strain (Rhodococcus) may be utilized for bioremediation of endosulfan.
从印度蚯蚓（Metaphire posthuma）肠道中分离出的细菌 Rhodococcus MTCC 6716 具有代谢硫丹的潜力，本研究旨在探讨其用于硫丹生物修复的可能性。本工作研究了最大生长、硫丹降解动力学、回归方程、半衰期和相关系数的最佳条件。还研究了硫丹诱导特定硫丹代谢标记基因（Esd）的 mRNA 和蛋白质表达的变化。在液体培养基中，细菌的最大生长在 pH 7.0、30°C 和 0.085 M 氯化钠浓度下观察到。在 15 天内，硫丹被 Rhodococcus 菌株降解了 97.23%，没有产生有毒代谢物，且与强相关系数（-0.728）和半衰期 5.99 天有关。硫丹的降解是通过基因组 DNA 中存在的基因（s）介导的。标记基因的表达与硫丹浓度有关。这些结果表明，这种新型菌株（Rhodococcus）可能可用于硫丹的生物修复。
