Licenciatura en Ingeniería Biológica, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City, Mexico.
Depto. de Ingeniería Agrícola y uso de la tierra, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentine.
Folia Microbiol (Praha). 2021 Dec;66(6):973-981. doi: 10.1007/s12223-021-00899-5. Epub 2021 Jul 14.
Endosulfan is an organochlorine pesticide included in the Stockholm Convention for Persistent Organic Compounds. The utilization of endosulfan as the sole source of carbon and its mineralization was evaluated using pure strains of Bacillus subtilis, Bacillus pseudomycoides, Peribacillus simplex, Enterobacter cloacae, Achromobacter spanius, and Pseudomonas putida, isolated from soil with historical pesticide use. The consumption of the α isomer of endosulfan by five of the six strains studied was higher than 95%, while B. subtilis degraded only 76% of the initial concentration (14 mg/L). On the other hand, the degradation of the β isomer was approximately 86% of the initial concentration (6 mg/L) by B. subtilis, P. simplex, and B. pseudomycoides and 95% by P. putida, E. cloacae, and A. spanius. The ability of A. spanius, P. simplex, and B. pseudomycoides to degrade endosulfan has not been previously reported. The production of endosulfan lactone by the Bacillus strains, as well as A. spanius and P. putida, indicated that endosulfan was degraded by the hydrolytic pathway.
硫丹是一种有机氯农药，被列入《关于持久性有机污染物的斯德哥尔摩公约》。本研究采用从历史上使用过农药的土壤中分离出的枯草芽孢杆菌、蕈状芽孢杆菌、简单芽孢杆菌、阴沟肠杆菌、黄杆菌和恶臭假单胞菌等纯培养物，评估了将硫丹作为唯一碳源加以利用及其矿化情况。在所研究的 6 株菌中，有 5 株菌对硫丹的α异构体的消耗率高于 95%，而枯草芽孢杆菌仅能降解初始浓度（14mg/L）的 76%。另一方面，枯草芽孢杆菌、简单芽孢杆菌和蕈状芽孢杆菌能将β异构体降解至初始浓度的约 86%（6mg/L），而恶臭假单胞菌、阴沟肠杆菌和黄杆菌则能将其降解 95%。此前尚未有报道称黄杆菌、简单芽孢杆菌和蕈状芽孢杆菌具有降解硫丹的能力。枯草芽孢杆菌以及黄杆菌和恶臭假单胞菌产生硫丹内酯表明，硫丹是通过水解途径进行降解的。
