用于白色念珠菌和念珠地丝菌鉴别的间接免疫荧光染色和交叉免疫电泳
Indirect immunofluorescence staining and crossed immunoelectrophoresis for differentiation of Candida albicans and Geotrichum candidum.
作者信息
Jensen H E, Hau J, Aalbaek B, Schønheyder H
机构信息
Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Frederiksberg, Denmark.
出版信息
Mycoses. 1990 Nov-Dec;33(11-12):519-26. doi: 10.1111/myc.1990.33.11-12.519.
Rabbit hyperimmune antisera were used for differential diagnosis of Candida albicans and Geotrichum candidum in murine and bovine tissues by indirect immunofluorescence (IIF) staining. Although the antisera did not detect common antigens by crossed immunoelectrophoresis (XIE) a marked cross-reactivity of fungal cells was observed in the IIF assay. Following liquid phase absorption with heterologous antigenic preparations the hyperimmune antisera were rendered specific for each species thus making possible an unequivocal differentiation of the two species.
兔超免疫抗血清用于通过间接免疫荧光（IIF）染色对小鼠和牛组织中的白色念珠菌和念珠地丝菌进行鉴别诊断。尽管抗血清通过交叉免疫电泳（XIE）未检测到共同抗原，但在IIF试验中观察到真菌细胞有明显的交叉反应性。用异源抗原制剂进行液相吸收后，超免疫抗血清对每个物种具有特异性，从而能够明确区分这两个物种。
Zotero 插件