Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, College of Chemistry and Material Science, Shaanxi Normal University, Xi'an, China.
Biochem Biophys Res Commun. 2010 Nov 26;402(4):773-7. doi: 10.1016/j.bbrc.2010.10.108. Epub 2010 Oct 29.
Melamine is a widely-used chemical in industries. In recent years, melamine has been found to be involved in outbreaks of renal injury in infants and animals. Pathological studies indicated that the melamine-induced acute renal failure was related to the concurrence of melamine and other triazine analogs such as cyanuric acid. In the present study, human erythrocytes were used as an in vitro model to explore the cytotoxicity of melamine and its complex with cyanuric acid. The results demonstrated that mixing melamine and cyanuric acid resulted in the formation of insoluble particles and that the insoluble melamine-cyanurate complex induced membrane damages of human erythrocytes. The membrane damages included hemolysis, K(+) leakage, alterations in cell shape and membrane fragility, and inhibition of enzymatic activity. By contrast, either melamine or cyanuric acid alone had no effect on erythrocyte membranes. The results of this study may provide a fresh insight into the melamine toxicology.
三聚氰胺是一种广泛应用于工业的化学物质。近年来，三聚氰胺已被发现与婴儿和动物的肾损伤爆发有关。病理学研究表明，三聚氰胺引起的急性肾衰竭与三聚氰胺与其他三嗪类似物（如三聚氰酸）的共存有关。在本研究中，用人红细胞作为体外模型来探讨三聚氰胺及其与三聚氰酸的复合物的细胞毒性。结果表明，三聚氰胺和三聚氰酸混合会形成不溶性颗粒，而不溶性三聚氰胺-三聚氰酸复合物会导致人红细胞的膜损伤。膜损伤包括溶血、K+泄漏、细胞形状和膜脆性的改变以及酶活性的抑制。相比之下，三聚氰胺或三聚氰酸单独作用于红细胞膜时无影响。本研究结果可能为三聚氰胺毒理学提供新的见解。
