进一步研究狒狒的巴雷特黏膜：化生腺细胞产生唾液酸性黏蛋白。

Further studies on Barretts mucosa in baboons: metaplastic glandular cells produce sialomucin.

机构信息

Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX, 78245-0549, USA.

出版信息

Anticancer Res. 2010 Oct;30(10):4123-6.

PMID:21036729

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3468319/

Abstract

BACKGROUND

In humans and in baboons, protracted gastro-esophageal reflux (GER) transforms the squamous-lined esophagus into columnar-lined (that is Barrett's mucosa, BM). Alcian blue stain (AB) is used to evidence sialomucin-producing goblet cells in human BM.

AIM

To assess the frequency and distribution of sialomucin-producing cells in BM in baboons.

MATERIALS AND METHODS

Sections from 137 consecutive baboon esophagi were alternatively stained with hematoxylin-eosin (H&E) and with AB (pH 2.5), without counterstain.

RESULTS

Out of 137 baboons, 131 (95.6%) had BM. Columnar and intramucosal glandular cells produced sialomucin in all 131 of these animals. Many BM cells were ballooned and filled with sialomucins, despite goblet cells not being found in H&E sections.

CONCLUSION

In humans, protracted GER is a disease requiring medication that may lead to BM; AB stains mainly goblet cells and occasional columnar cells in BM. In baboons, in contrast, BM is a natural postnatal process of adaptation to GER, triggered by regurgitation and rumination. AB stains all columnar and intra-mucosal glandular cells. Sialomucin-overstuffed cells were more frequent and larger in baboons than in humans. The extra load of sialomucin in BM might be an integrated part of the postnatal life-long process of adaptation to regurgitation and rumination in baboons.

摘要

背景

在人类和狒狒中，长时间的胃食管反流（GER）会使鳞状 lined 食管转变为柱状 lined（即 Barrett 黏膜，BM）。使用阿尔辛蓝染色（AB）来证明人类 BM 中的分泌唾液粘蛋白的杯状细胞。

目的

评估狒狒 BM 中分泌唾液粘蛋白的细胞的频率和分布。

材料和方法

对 137 例连续的狒狒食管切片进行交替染色，包括苏木精-伊红（H&E）和 AB（pH 2.5）染色，不进行复染。

结果

在 137 例狒狒中，有 131 例（95.6%）存在 BM。柱状和黏膜内腺细胞在所有这 131 例动物中均产生唾液粘蛋白。尽管在 H&E 切片中未发现杯状细胞，但许多 BM 细胞呈气球样并充满了唾液粘蛋白。

结论

在人类中，长时间的 GER 是一种需要药物治疗的疾病，可能导致 BM；AB 染色主要染色 BM 中的杯状细胞和偶尔的柱状细胞。相比之下，在狒狒中，BM 是一种对 GER 的自然产后适应过程，由反流和反刍触发。AB 染色所有柱状和黏膜内腺细胞。在狒狒中，唾液粘蛋白过载的细胞比在人类中更频繁且更大。BM 中唾液粘蛋白的额外负荷可能是狒狒对反流和反刍进行产后终身适应过程的一个组成部分。