Department of Ophthalmology, Far Eastern Memorial Hospital, Ban-Chiao, Taipei, Taiwan, Republic of China.
Br J Ophthalmol. 2011 Feb;95(2):277-83. doi: 10.1136/bjo.2010.189407. Epub 2010 Oct 29.
BACKGROUND/AIMS: Povidone-iodine (PI) is commonly used as a preoperative disinfectant; however, it has been shown to be cytotoxic. The present study was performed to investigate the mechanism by which PI causes cell death.
Primary human corneal fibroblasts (HCF) and a human corneal epithelial cell line (HCEC) were treated with 0.1-5% PI for 1 min. Cell morphology and growth were examined by phase-contrast microscopy and genomic DNA quantification. Cellular enzyme activities were detected by water-soluble tetrazolium salt (WST-1) and calcein-acetoxymethylester staining, whereas membrane integrity was determined by a membrane-impermeable dye. The cell fixation effect of PI was assayed by analysis of genomic DNA integrity and resistance to ionic detergent SDS lysis. The interleukin-8 (IL-8) secretion after adding interleukin-1ß (IL-1b) or lipopolysaccharide (LPS) was determined by ELISA.
PI treatment inhibited HCF and HCEC cell growth without changing cellular morphology; however, cells became resistant to SDS lysis. The mitochondrial dehydrogenase and intracellular esterase activities as well as cell membrane integrity were abolished by PI treatment. Genomic DNA integrity from PI-treated groups was similar to that from alcohol-fixed groups. IL-1b- and LPS-induced IL-8 secretion was abolished by PI treatment.
Where PI concentration is sufficient to cause cell death, this occurs through fixation rather than necrosis in cultured human corneal stromal and epithelial cell.
背景/目的：聚维酮碘（PI）通常用作术前消毒剂；然而，它已被证明具有细胞毒性。本研究旨在探讨 PI 导致细胞死亡的机制。
用人眼角膜成纤维细胞（HCF）和人眼角膜上皮细胞系（HCEC）分别用 0.1-5%的 PI 处理 1 分钟。通过相差显微镜和基因组 DNA 定量检测细胞形态和生长。通过水溶性四唑盐（WST-1）和钙黄绿素-乙酰氧甲酯染色检测细胞酶活性，通过不透膜染料检测膜完整性。通过分析基因组 DNA 完整性和对离子去污剂 SDS 裂解的抗性来检测 PI 的细胞固定作用。通过 ELISA 测定加入白细胞介素-1β（IL-1β）或脂多糖（LPS）后白细胞介素-8（IL-8）的分泌。
PI 处理抑制 HCF 和 HCEC 细胞生长，而不改变细胞形态；然而，细胞对 SDS 裂解的抗性增强。线粒体脱氢酶和细胞内酯酶活性以及细胞膜完整性被 PI 处理所破坏。PI 处理组的基因组 DNA 完整性与酒精固定组相似。PI 处理可消除 IL-1β和 LPS 诱导的 IL-8 分泌。
在 PI 浓度足以导致细胞死亡的情况下，这种死亡是通过固定而不是坏死发生在培养的人角膜基质和上皮细胞中。
