Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand.
Am J Trop Med Hyg. 2010 Nov;83(5):1005-9. doi: 10.4269/ajtmh.2010.10-0339.
We evaluated the influence of pfmdr1 mutations and copy number on in vitro artemether and lumefantrine sensitivity in 101 laboratory and adapted Thai isolates of Plasmodium falciparum. Approximately one-fourth of these isolates exhibited reduced lumefantrine susceptibility. We found that both mutations and amplification of the pfmdr1 gene influenced in vitro artemether and lumefantrine sensitivity. Using multivariate analysis, 184F or 1042N alleles and a copy number of ≥ 4 were identified as the independent markers for decreased lumefantrine susceptibility. Separate analysis also indicated that parasites from different geographical areas were influenced by different genetic markers.
我们评估了 pfmdr1 突变和拷贝数对 101 株实验室和泰国适应性疟原虫 falciparum 分离株体外青蒿素和咯萘啶敏感性的影响。大约四分之一的这些分离株显示出咯萘啶敏感性降低。我们发现 pfmdr1 基因的突变和扩增均影响青蒿素和咯萘啶的体外敏感性。多变量分析显示，184F 或 1042N 等位基因和拷贝数≥4 是咯萘啶敏感性降低的独立标志物。单独分析还表明，来自不同地理区域的寄生虫受到不同遗传标记的影响。
