犬猫临床样本中存在具有分子误诊可能性的机会性细菌（根瘤菌属）。
Presence of opportunistic bacteria (Rhizobium spp.) with potential for molecular misdiagnosis among canine and feline clinical samples.
作者信息
Tabar Maria-Dolores, Altet Laura, Roura Xavier, Sánchez Armand, Ferrer Lluís, Francino Olga
机构信息
Veterinary Teaching Hospital, Autonomous University of Barcelona, Barcelona 08193, Spain.
出版信息
Can Vet J. 2010 Aug;51(8):895-7.
Abstract
Rhizobium radiobacter was detected in 12 of 187 dogs and 2 of 100 cats using a polymerase chain reaction (PCR) assay formerly designed for the Rickettsia genus. Although PCR primers used for pathogenic infectious agents are specifically assessed to avoid cross-amplification, this retrospective study highlights the importance of sequencing to avoid molecular misdiagnosis.
摘要
使用先前针对立克次氏体属设计的聚合酶链反应（PCR）检测方法，在187只狗中的12只以及100只猫中的2只身上检测到了根癌土壤杆菌。尽管用于病原性感染因子的PCR引物经过专门评估以避免交叉扩增，但这项回顾性研究凸显了测序对于避免分子误诊的重要性。
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