Ding T, Jiang T, Zhou J, Xu L, Gao Z M
School of Plant Protection, Anhui Agricultural University, Hefei, China.
Genet Mol Res. 2010 Oct 26;9(4):2104-12. doi: 10.4238/vol9-4gmr809.
Agricultural research of plant-derived endophytic fungi has grown in recent decades. We isolated 26 endophytic fungi from the leaves, stems and fruits of "the tree of life", Camptotheca acuminata, and tested them for antimicrobial activities based on growth inhibition measurements in a modified agar diffusion method. Fermentation broths from most of the isolates exhibited antifungal activity and 50% exhibited antibacterial activity; some of them also exhibited strong broad-spectrum antimicrobial activity. The strongest antimicrobial activity was exhibited by strains XSY10 and XSY15 against Rhizoctonia solani and Fusarium oxysporum f. sp. vasinfectum, with 75% and 67% inhibition, respectively. Strain XSJ01 gave strong activity against pathogenic bacteria, with inhibition zones more than 20 mm in diameter. The isolates were identified by molecular methods as belonging to nine taxa: Nigrospora, Diaporthe, Alternaria, Colletotrichum, Pestalotiopsis, Sordariomycete, Guignardiai, Penicillium, and Zythia. Based on these results, we conclude that the endophytic fungi of C. acuminata are promising sources of novel bioactive compounds.
近几十年来，对植物源内生真菌的农业研究不断发展。我们从“生命之树”喜树的叶、茎和果实中分离出26种内生真菌，并采用改良的琼脂扩散法通过生长抑制测量来测试它们的抗菌活性。大多数分离株的发酵液表现出抗真菌活性，50%表现出抗菌活性；其中一些还表现出强大的广谱抗菌活性。菌株XSY10和XSY15对立枯丝核菌和尖孢镰刀菌黄瓜专化型表现出最强的抗菌活性，抑制率分别为75%和67%。菌株XSJ01对病原菌有很强的活性，抑菌圈直径超过20毫米。通过分子方法将这些分离株鉴定为属于九个分类单元：黑孢霉属、间座壳属、链格孢属、炭疽菌属、拟盘多毛孢属、粪壳菌纲、吉氏霉属、青霉属和齐氏酵母属。基于这些结果，我们得出结论，喜树内生真菌是新型生物活性化合物的有前途的来源。
