Luo Ze-Ping, Lin Hai-Yan, Ding Wen-Bing, He Hua-Liang, Li You-Zhi
Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Hunan Agricultural University, Changsha 410128, China.; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China.
Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China.; National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China.
Mycobiology. 2015 Dec;43(4):435-43. doi: 10.5941/MYCO.2015.43.4.435. Epub 2015 Dec 31.
Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.
从紫穗槐的叶、茎和根中分离出61株具有不同菌落形态的内生真菌菌株，其定殖率分别为66.95%、37.50%和26.92%。基于内转录间隔区序列分析，61个分离株被归类为子囊菌门3个纲的16个属。根据对峙培养试验，61个分离株中有6株（9.84%）对一种或多种指示植物病原真菌表现出抗真菌活性。分离株TPL25具有最广的抗真菌活性谱，分离株TPL35对5种植物病原真菌有活性。此外，TPL25和TPL35的培养滤液对核盘菌的生长抑制率大于80%。我们得出结论，内生真菌菌株TPL25和TPL35是有前景的生物活性化合物来源。
