碳酸酐酶 XII 的表达与宫颈癌的组织学分级相关，并与放疗效果较好相关。

BACKGROUND

To investigate whether expression of carbonic anhydrase XII (CA12) is associated with histologic grade of the tumors and radiotherapy outcomes of the patients with invasive cervical cancer.

METHODS

CA12 expression was examined by immunohistochemical stains in cervical cancer tissues from 183 radiotherapy patients. Histological grading was classified as well (WD), moderately (MD) or poorly differentiated (PD). Oligonucleotide microarray experiment was performed using seven cervical cancer samples to examine differentially expressed genes between WD and PD cervical cancers. The association between CA12 and histological grade was analyzed by chi-square test. CA12 and histological grades were analyzed individually and as combined CA12 and histologic grade categories for effects on survival outcome.

RESULTS

Immunohistochemical expression of CA12 was highly associated with the histologic grade of cervical cancer. Lack of CA12 expression was associated with PD histology, with an odds ratio of 3.9 (P = 0.01). Microarray analysis showed a fourfold reduction in CA12 gene expression in PD tumors. CA12 expression was marginally associated with superior disease-free survival. Application of the new combined categories resulted in further discrimination of the prognosis of patients with moderate and poorly differentiated tumor grade.

CONCLUSIONS

Our study indicates that CA12 may be used as a novel prognostic marker in combination with histologic grade of the tumors.

背景

研究碳酸酐酶 XII（CA12）的表达是否与浸润性宫颈癌患者的肿瘤组织学分级和放疗结果有关。

方法

对 183 例接受放疗的宫颈癌患者的组织标本进行 CA12 免疫组化染色。组织学分级分为高分化（WD）、中分化（MD）或低分化（PD）。使用七种宫颈癌样本进行寡核苷酸微阵列实验，以检查 WD 和 PD 宫颈癌之间差异表达的基因。通过卡方检验分析 CA12 与组织学分级之间的关系。分别分析 CA12 和组织学分级以及 CA12 和组织学分级联合类别对生存结果的影响。