Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Ponte don Melillo, 84084 Fisciano (SA), Italy.
J Pharm Biomed Anal. 2011 Feb 20;54(3):535-44. doi: 10.1016/j.jpba.2010.10.004. Epub 2010 Oct 14.
Liquid chromatography electrospray mass spectrometry (LC-ESI/MS) has been applied to the full characterization of saponins and phenolics in hydroalcoholic extracts of roots of liquorice (Glycyrrhiza glabra). Relative quantitative analyses of the samples with respect to the phenolic constituents and to a group of saponins related to glycyrrhizic acid were performed using LC-ESI/MS. For the saponin constituents, full scan LC-MS/MS fragmentation of the protonated (positive ion mode) or deprotonated (negative ion mode) molecular species generated diagnostic fragment ions that provided information concerning the triterpene skeleton and the number and nature of the substituents. On the basis of the specific fragmentation of glycyrrhizic acid, an LC-MS/MS method was developed in order to quantify the analyte in the liquorice root samples. Chinese G. glabra roots contained the highest levels of glycyrrhizic acid, followed by those from Italy (Calabria).
液相色谱-电喷雾质谱（LC-ESI/MS）已被应用于甘草（Glycyrrhiza glabra）根的水醇提取物中皂苷和酚类物质的全面分析。采用 LC-ESI/MS 对样品中酚类成分和一组与甘草酸相关的皂苷进行了相对定量分析。对于皂苷成分，质子化（正离子模式）或去质子化（负离子模式）分子物种的全扫描 LC-MS/MS 碎裂生成了具有诊断意义的碎片离子，这些离子提供了有关三萜骨架和取代基数量和性质的信息。基于甘草酸的特异性碎裂，开发了一种 LC-MS/MS 方法，以便定量分析甘草根样品中的分析物。中国的甘草根中含有最高水平的甘草酸，其次是来自意大利（卡拉布里亚）的甘草根。
