全鼠冷冻成像中的颜色解剖学和分子荧光可视化。
Visualization of color anatomy and molecular fluorescence in whole-mouse cryo-imaging.
机构信息
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
出版信息
Comput Med Imaging Graph. 2011 Apr;35(3):195-205. doi: 10.1016/j.compmedimag.2010.10.003. Epub 2010 Oct 30.
Abstract
We developed multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications. Using true-color volume rendering, we interactively, selectively enhanced anatomy using feature detection. For example, to enhance red organs (vessels, liver, etc.) and internal surfaces, we computed a red feature from R/(R+G+B) and surface features from color/gray-scale gradients, respectively. For >70GB cryo-image volumes, we developed multi-resolution visualization, which provided low-resolution rendering of an entire mouse and zooming to organs, tissues, and cells. Fusions of fluorescence and color cryo-volumes uniquely showed biodistribution of metastatic and stem cells within an anatomical context.
摘要
我们开发了多尺度、实时交互式彩色图像数据可视化技术，包括适用于多种生物医学应用的显微镜全鼠冷冻图像。使用真彩色体绘制，我们通过特征检测来交互式、选择性地增强解剖结构。例如，为了增强红色器官（血管、肝脏等）和内部表面，我们分别从 R/(R+G+B) 中计算红色特征，并从颜色/灰度梯度中计算表面特征。对于超过 70GB 的冷冻图像体积，我们开发了多分辨率可视化技术，它提供了整个老鼠的低分辨率渲染，并可放大到器官、组织和细胞。荧光和彩色冷冻体积的融合独特地显示了转移和干细胞在解剖背景下的生物分布。
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