Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110, USA.
J Cell Biol. 2010 Nov 1;191(3):493-503. doi: 10.1083/jcb.201006092.
How dividing cells monitor the effective transmission of genomes during mitosis is poorly understood. Budding yeast use a signaling pathway known as the spindle position checkpoint (SPC) to ensure the arrival of one end of the mitotic spindle in the nascent daughter cell. An important question is how SPC activity is coordinated with mother-daughter polarity. We sought to identify factors at the bud neck, the junction between mother and bud, which contribute to checkpoint signaling. In this paper, we show that the protein kinase Elm1 is an obligate regulator of the SPC, and this function requires localization of Elm1 to the bud neck. Furthermore, we show that Elm1 promotes the activity of the checkpoint kinase Kin4. These findings reveal a novel function for Elm1 in the SPC and suggest how checkpoint activity may be linked to cellular organization.
细胞如何在有丝分裂过程中监测基因组的有效传递尚不清楚。 budding 酵母使用一种称为纺锤体位置检查点（SPC）的信号通路来确保有丝分裂纺锤体的一端进入新形成的子细胞。一个重要的问题是 SPC 活性如何与母-子极性相协调。我们试图确定芽颈（母细胞和芽之间的连接处）中的因子，这些因子有助于检查点信号传导。在本文中，我们表明蛋白激酶 Elm1 是 SPC 的必需调节剂，并且该功能需要 Elm1 定位到芽颈。此外，我们表明 Elm1 促进了检查点激酶 Kin4 的活性。这些发现揭示了 Elm1 在 SPC 中的新功能，并表明检查点活性如何与细胞组织相关联。
