Ozone Nanobubble Water as a Sustainable Strategy to Enhance Metabolism, Muscle Function, and Exercise Performance in Mice.

: Nanobubble water (NBW) is being studied increasingly for its potential benefits in sports nutrition. This study aimed to evaluate whether supplementation with ozone-enriched NBW (O-NBW) could improve integrated exercise capacity-encompassing endurance performance, muscle strength, and postexercise recovery as well as body composition and metabolic adaptations in mice. Male ICR mice ( = 24) were allocated into Control, Air-NBW, or O-NBW (0.2-1 mg/L ozone) groups for 4 weeks. O-NBW treatment considerably enhanced forelimb grip strength and treadmill running endurance compared to the Control group (both < 0.05). Analyses of body composition revealed a higher proportion of lean mass and muscle glycogen storage in NBW groups, notably with O-NBW. Serum markers gathered post-exercise demonstrated a reduction in ammonia and blood urea nitrogen (BUN), suggesting improved nitrogen metabolism. Levels of resting serum creatine kinase (CK) and uric acid were also lower in O-NBW mice, indicating potential benefits for muscle recovery. In addition, O-NBW treatment significantly enhanced oxygen consumption (VO) and reduced the respiratory quotient (RQ), signifying amplified fat oxidation, while also lowering total energy expenditure (all < 0.05). Spontaneous wheel-running activity remained consistent across all the groups. Taken as a whole, these findings emphasize that O-NBW supplementation offers ergogenic and metabolic advantages by improving integrated exercise capacity and efficiency of gas exchange, without adverse effects.