Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
Lasers Med Sci. 2011 Mar;26(2):257-65. doi: 10.1007/s10103-010-0849-y. Epub 2010 Nov 3.
Surgeries performed with high-intensity laser devices may be improved with accurate protocols, including the air-water spray regulation. Thus, this study sought to investigate the healing process of wounds made on the dorsum of rat tongues using an Er,Cr:YSGG laser device with different air-water spray regulations. The incisions were made on the dorsum of Wistar rat tongues using an Er,Cr:YSGG laser with three different air-water spray regulations (100/0%, 50/50%, 11/7%). Scalpel incisions functioned as controls. The sacrifices occurred between 0 and 14 days after surgery. Morphological, histological, and immunohistochemical (fibronectin and type III collagen) analysis of the wounds were performed. The air-water spray regulation influenced wound healing and the inflammatory response, especially in the earlier stages. Incisions performed using the 100/0% air/water spray regulation had the worst results, expressing a greater amount of fibronectin and type III collagen. The 50/50% air/water spray regulation brought in a non-clear surgical field and poor laser interaction with the tissue. The 11/7% air/water spray regulation showed the best clinical results and less pronounced histological events. According to the results encountered, the air-water spray should be regulated to improve surgery.
使用高强度激光设备进行的手术可以通过准确的方案得到改善，包括空气-水喷雾调节。因此，本研究旨在研究使用不同空气-水喷雾调节的 Er,Cr:YSGG 激光设备对大鼠舌背伤口的愈合过程。使用 Er,Cr:YSGG 激光在 Wistar 大鼠舌背上制造切口，采用三种不同的空气-水喷雾调节（100/0%、50/50%、11/7%）。手术刀切口作为对照。手术切除后 0 至 14 天进行牺牲。对伤口进行形态学、组织学和免疫组织化学（纤维连接蛋白和 III 型胶原）分析。空气-水喷雾调节影响伤口愈合和炎症反应，特别是在早期阶段。使用 100/0%空气/水喷雾调节进行的切口效果最差，表达了更多的纤维连接蛋白和 III 型胶原。50/50%空气/水喷雾调节带来不清晰的手术视野和激光与组织的不良相互作用。11/7%空气/水喷雾调节显示出最佳的临床效果和不太明显的组织学事件。根据遇到的结果，应调节空气-水喷雾以改善手术。
