Physiology Department, National Institute of Cardiology "Ignacio Chávez". Juan Badiano # 1, Col. Sección XVI, Tlalpan, C.P. 14080 México, D.F., México.
Diabetol Metab Syndr. 2010 Nov 2;2:64. doi: 10.1186/1758-5996-2-64.
Metabolic syndrome (MS) may comprise several clinical conditions such as obesity, diabetes and inflammatory disorders, which are characterized by metabolic imbalances. The study of glucose transport and regulation by insulin in lymphocytes is important, since the way they increase inflammation and susceptibility to infections are common in MS. We studied glucose internalization in isolated thymocytes and splenocytes, its regulation by insulin, and the role of three glucose transporters (Gluts) in control and in MS rats. Control glucose internalization and insulin responses were lower in splenocytes than in thymocytes. Control and insulin-induced glucose internalization in thymocytes declined with age, while transport by splenocyte continued to respond to insulin. Control thymocyte glucose internalization was blocked by antibodies against Glut 1 and 4, while the insulin response also was blocked by an anti-Glut 3 antibody. On four month old control and insulin-induced response, splenocyte transport was only blocked by Glut 1 and 4 antibodies. At six months splenocyte glucose internalization depended on Glut 1 and was less sensitive to the effects of an anti-Glut 4 antibody. In MS splenocytes the capacity of anti-Glut 1 antibodies to inhibit control and insulin-dependent glucose transport was less significant, and we found that in MS rats, glucose internalization was dependent on Glut 3 and Glut 4. In summary, the altered metabolic state present in MS rats shows signs of modulation of glucose internalization by the Glut1, Glut 3 and Glut 4 transporters, compared with its own age control.
代谢综合征（MS）可能包括肥胖、糖尿病和炎症性疾病等几种临床病症，这些病症的特征是代谢失衡。研究淋巴细胞中葡萄糖的转运和胰岛素调节非常重要，因为它们增加炎症和易感染的方式在 MS 中很常见。我们研究了分离的胸腺细胞和脾细胞中葡萄糖的内化及其胰岛素调节，以及三种葡萄糖转运体（Glut）在对照和 MS 大鼠中的作用。与胸腺细胞相比，脾细胞中葡萄糖的内化和胰岛素反应较低。控制和胰岛素诱导的胸腺细胞葡萄糖内化随年龄增长而下降，而脾细胞的转运仍对胰岛素有反应。对照胸腺细胞葡萄糖内化被针对 Glut1 和 4 的抗体阻断，而胰岛素反应也被针对 Glut3 的抗体阻断。在四个月大的对照和胰岛素诱导反应中，脾细胞转运仅被 Glut1 和 4 抗体阻断。在六个月时，脾细胞葡萄糖内化依赖于 Glut1，对 Glut4 抗体的作用不那么敏感。在 MS 脾细胞中，抗 Glut1 抗体抑制对照和胰岛素依赖的葡萄糖转运的能力不那么显著，我们发现 MS 大鼠的葡萄糖内化依赖于 Glut3 和 Glut4。综上所述，与自身的年龄对照相比，MS 大鼠中存在的改变的代谢状态显示出葡萄糖内化受 Glut1、Glut3 和 Glut4 转运体调节的迹象。
