改良的快速酶微板测定法，用于定量细菌细胞内γ-氨基丁酸和琥珀酸半醛。
A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells.
机构信息
Bacterial Stress Response Group, Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland.
出版信息
J Microbiol Methods. 2011 Jan;84(1):137-9. doi: 10.1016/j.mimet.2010.10.017. Epub 2010 Oct 31.
PMID:21044648
Abstract
The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.
摘要
GABase 分析广泛用于快速准确地定量细胞外 γ-氨基丁酸 (GABA) 的水平。在这里，我们展示了该分析的一种改良方法，使我们能够定量细菌细胞内的 GABA。细胞通过煮沸和乙醇胺-O-硫酸盐裂解，使用 GABA 转氨酶抑制剂来区分 GABA 和琥珀酸半醛。
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