Department of Materials Science and Engineering, National Chiao-Tung University, Hsinchu 300, Taiwan.
Biosens Bioelectron. 2011 Jan 15;26(5):2413-8. doi: 10.1016/j.bios.2010.10.022. Epub 2010 Oct 16.
Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10 nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.
使用自组装的银纳米粒子（AgNPs）修饰的金纳米尖（AuNT）阵列，在亚皮摩尔水平上实现了对腺嘌呤和胸腺嘧啶寡核苷酸的无标记光学传感。该平台由 AuNTs 组成，不仅有助于高效的生物固定化，而且将 AgNPs 包装在三维高表面积工作空间中，有助于表面增强拉曼散射（SERS）。使用亚 10nm 的 AgNPs 并确保最佳的颗粒间距离，可确保在 SERS 实验中吸附的腺嘌呤、胸腺嘧啶、胞嘧啶和鸟嘌呤分子的化学特异性拉曼信号得到放大。高时间稳定性的拉曼信号确保了可靠和可重复的 DNA 检测，即使在三周的环境台式保存后也是如此。这种简便的架构是三维的且非光刻的，与传统的 SERS 平台不同。
