非长末端重复序列转座子 LINE-1 的 ORF1p 的核酸伴侣特性。
Nucleic acid chaperone properties of ORF1p from the non-LTR retrotransposon, LINE-1.
机构信息
Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO, USA.
出版信息
RNA Biol. 2010 Nov-Dec;7(6):706-11. doi: 10.4161/rna.7.6.13766. Epub 2010 Nov 1.
Abstract
Long interspersed element-1 (LINE-1, or L1) is a non-long terminal repeat (LTR) retrotransposon that has amplified to hundreds of thousands of copies in mammalian evolution. A small number of the individual copies of L1 are active retrotransposons which are presently replicating in most species, including humans and mice. L1 retrotransposition begins with transcription of an active element and ends with a newly inserted cDNA copy, a process which requires the two element-encoded proteins to act in cis on the L1 RNA. The ORF1 protein (ORF1p) is a high-affinity, non-sequence-specific RNA binding protein with nucleic acid chaperone activity, whereas the ORF2 protein (ORF2p) supplies the enzymatic activities for cDNA synthesis. This article reviews the nucleic acid chaperone properties of ORF1p in the context of L1 retrotransposition.
摘要
长散布元件 1（LINE-1，或 L1）是非长末端重复（LTR）逆转录转座子，在哺乳动物进化过程中已扩增到数十万份。L1 的一小部分个体拷贝是活跃的逆转录转座子，目前在大多数物种中都在复制，包括人类和小鼠。L1 逆转录转座始于活性元件的转录，最后以新插入的 cDNA 拷贝结束，该过程需要两个元件编码的蛋白质在 cis 上作用于 L1 RNA。ORF1 蛋白（ORF1p）是一种具有核酸伴侣活性的高亲和力、非序列特异性 RNA 结合蛋白，而 ORF2 蛋白（ORF2p）则提供 cDNA 合成的酶活性。本文综述了 ORF1p 在 L1 逆转录转座过程中的核酸伴侣特性。
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