Department of Anatomy II and Cell Biology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Histochem Cell Biol. 2010 Dec;134(6):565-79. doi: 10.1007/s00418-010-0752-4. Epub 2010 Nov 3.
The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.
通过免疫组织化学显示，视网膜中存在线粒体脂肪酸β氧化系统。脂肪酸被认为是全身器官中由脂肪酸β氧化代谢和糖酵解代谢的主要能量来源，但关于视网膜中的这种代谢系统几乎一无所知。对成年大鼠视网膜进行免疫荧光和免疫电镜检查，以定位脂肪酸β氧化酶，同时进行 Western blot 分析以定量酶蛋白的量和 DNA 微阵列分析以检测基因表达。检查的所有酶都被证明存在于视网膜中，但数量很少，视网膜中的蛋白质和基因表达量约为肝脏的 1/10。光镜和电镜下的免疫组织化学显示，这些酶更优先定位于 Müller 细胞的线粒体，而不是视网膜神经元。从视网膜中分离出 Müller 细胞，并证实其存在线粒体脂肪酸β氧化酶。因此，证明了视网膜中存在异质性的线粒体脂肪酸β氧化系统。
