Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.
Biotechnol Lett. 2011 Mar;33(3):517-23. doi: 10.1007/s10529-010-0450-2. Epub 2010 Nov 3.
Although biofilms produced by various Leuconostoc sp. are economically important as contaminants of sugar processing plants, very few studies are available on these systems. Twelve strains of Leuconostoc citreum and L. mesenteroides that produce a variety of extracellular glucans were compared for their capacity to produce biofilms. 16s rRNA sequence analysis was used to confirm the species identity of these strains, which included four isolates of L. mesenteroides, five isolates of L. citreum, and three glucansucrase mutants of L. citreum strain NRRL B-1355. Strains identified as L. mesenteroides produce glucans that are generally similar to commercial dextran. Nevertheless, these strains differed widely in their capacity to form biofilms, with densities ranging from 2.7 to 6.1 log cfu/cm(2). L. citreum strains and their derivatives produce a variety of glucans. These strains exhibited biofilm densities ranging from 2.5 to 5.9 log cfu/cm(2). Thus, biofilm-forming capacity varied widely on a strain-specific basis in both species. The types of polysaccharides produced did not appear to affect the ability to form biofilms.
虽然各种肠膜明串珠菌产生的生物膜作为糖加工厂的污染物具有重要的经济意义，但对这些系统的研究却很少。对产生各种胞外葡聚糖的 12 株肠膜明串珠菌和肠膜状明串珠菌进行了比较，以研究它们形成生物膜的能力。16s rRNA 序列分析用于确认这些菌株的种属身份，其中包括 4 株肠膜状明串珠菌、5 株肠膜明串珠菌和 3 株肠膜明串珠菌 NRRL B-1355 的葡聚糖蔗糖酶突变株。鉴定为肠膜状明串珠菌的菌株产生的葡聚糖通常与商业右旋糖酐相似。然而，这些菌株形成生物膜的能力差异很大，密度范围从 2.7 到 6.1 log cfu/cm(2)。肠膜明串珠菌及其衍生物产生多种葡聚糖。这些菌株的生物膜密度范围为 2.5 至 5.9 log cfu/cm(2)。因此，两种菌的菌株特异性生物膜形成能力差异很大。所产生的多糖类型似乎并不影响形成生物膜的能力。
