College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China.
Sci China Life Sci. 2010 Nov;53(11):1307-14. doi: 10.1007/s11427-010-4078-1. Epub 2010 Nov 3.
In this study, we show that CIPK14, a stress responsive CBL-interacting protein kinase gene, is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis seedlings. The CIPK14-impairment mutant cipk14 grown in continuous far-red (FR) light did not show greening when exposed to white light illumination for 15 h. By contrast, the FR-grown phytochrome A null mutant phyA greened within 0.5 h of exposure to white light. Although greening of Col-4 (wild-type) was not completely abolished by FR, it exhibited a significantly decreased greening capacity compared with that of phyA. Further analyses demonstrated that the expression of protochlorophyllide reductase (POR) genes was correlated with the greening ability of the genotypes. In addition, CIPK14 appeared to be regulated by both the circadian clock and PhyA. Taken together, these results suggest that CIPK14 plays a role in PhyA-mediated FR inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway.
在这项研究中，我们表明，CIPK14，一种应激响应的 CBL 相互作用蛋白激酶基因，参与了拟南芥幼苗中光敏色素 A 介导的远红光抑制绿素的形成。在连续远红光（FR）光下生长的 CIPK14 缺陷突变体 cipk14 在暴露于白光照射 15 小时后没有表现出绿素。相比之下，phyA 缺失突变体在暴露于白光 0.5 小时内就出现了绿素。虽然 Col-4（野生型）的绿素形成没有被 FR 完全消除，但与 phyA 相比，其绿素形成能力明显降低。进一步的分析表明，原叶绿素还原酶（POR）基因的表达与基因型的绿素形成能力相关。此外，CIPK14 似乎受到生物钟和 PhyA 的双重调节。综上所述，这些结果表明，CIPK14 在 PhyA 介导的 FR 抑制幼苗绿素形成中发挥作用，并且钙相关激酶可能参与了光敏色素 A 信号通路中一个以前未定义的分支点。
